Lyne J E, Carter D C, He X M, Stubbs G, Hash J H
Space Science Laboratory, Marshall Space Flight Center, Huntsville, Alabama 35812.
J Biol Chem. 1990 Apr 25;265(12):6928-30.
The lysozyme from the fungus of the Chalaropsis species has been crystallized. This lysozyme (Mr 22,415) displays no sequence homology with avian, phage, or mammalian lysozymes, however, preliminary studies indicate significant sequence homology with the bacterial lysozyme from Streptomyces. Both enzymes are unusual in possessing beta-1,4-N-acetylmuramidase and beta-1,4-N,6-O-diacetylmuramidase activity. The crystals grow from solutions of ammonium sulfate during growth periods from several months to a year. The space group is P2(1)2(1)2(1) with a = 34.0 A, b = 42.6 A, c = 122.1 A. Preliminary data indicate that there is 1 molecule/asymmetric unit. A complete native data set has been collected to 2.57-A resolution. The crystals are highly ordered and exhibit diffraction patterns to d-spacings less than 1.5 A.
嗜热栖热放线菌属真菌的溶菌酶已被结晶。这种溶菌酶（分子量22,415）与鸟类、噬菌体或哺乳动物的溶菌酶没有序列同源性，然而，初步研究表明它与链霉菌属的细菌溶菌酶有显著的序列同源性。这两种酶的不同寻常之处在于都具有β-1,4-N-乙酰胞壁酸酶和β-1,4-N,6-O-二乙酰胞壁酸酶活性。晶体在数月至一年的生长期间从硫酸铵溶液中生长出来。空间群为P2(1)2(1)2(1)，a = 34.0 Å，b = 42.6 Å，c = 122.1 Å。初步数据表明每个不对称单位有1个分子。已收集到分辨率为2.57 Å的完整天然数据集。晶体高度有序，其衍射图谱的d间距小于1.5 Å。
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