哈茨木霉木聚糖酶的结晶及初步X射线衍射研究

Crystallization and preliminary X-ray diffraction study of a xylanase from Trichoderma harzianum.

作者信息

Rose D R, Birnbaum G I, Tan L U, Saddler J N

机构信息

Division of Biological Sciences, National Research Council Ottawa, Ontario, Canada.

出版信息

J Mol Biol. 1987 Apr 20;194(4):755-6. doi: 10.1016/0022-2836(87)90254-3.

DOI:10.1016/0022-2836(87)90254-3

PMID:3656407

Abstract

A 20,000 Mr xylanase from Trichoderma harzianum has been purified and crystallized from 20% (w/v) saturated ammonium sulphate solutions. The unit cell is orthorhombic, space group P2(1)2(1)2(1), with unit cell lengths a = 44.2 A, b = 94.1 A, c = 51.6 A. Data from native crystals and several potential heavy-atom derivatives have been collected. An X-ray analysis to at least 2.8 A resolution appears to be feasible.

摘要

一种来自哈茨木霉的20000道尔顿木聚糖酶已从20%（w/v）饱和硫酸铵溶液中纯化并结晶。晶胞为正交晶系，空间群P2(1)2(1)2(1)，晶胞参数a = 44.2 Å，b = 94.1 Å，c = 51.6 Å。已收集了天然晶体和几种潜在重原子衍生物的数据。进行至少2.8 Å分辨率的X射线分析似乎是可行的。