Cunningham E T, Bohn M C, Sawchenko P E
Department of Neuroscience, University of California, San Diego.
J Comp Neurol. 1990 Feb 22;292(4):651-67. doi: 10.1002/cne.902920413.
Anterograde transport, retrograde transport, and immunohistochemical techniques were used to characterize the organization of neural inputs to the paraventricular (PVH) and supraoptic (SO) nuclei from the C1, C2, and C3 adrenergic cell groups in the rostral medulla. The results are as follows: 1) Phenylethanolamine-N-methyltransferase-immunoreactive (PNMT-IR) fibers and terminals were distributed to all parts of the parvicellular division of the PVH; the dorsal and dorsal medial subdivisions received the most prominent inputs, the lateral and ventral medial parts the least. Sparse terminal fields were found consistently in the magnocellular division of the PVH and in the SO. 2) A combined retrograde transport-immunohistochemical method was used to estimate the number and proportion of cells in the regions of the C1, C2, and C3 cell groups that contribute to the PNMT-IR innervation of the PVH. On average, 232 +/- 37 retrogradely labeled cells in the C1 cell group, 73 +/- 32 in the C2 cell group, and 96 +/- 26 in the C3 group stained positively for PNMT-IR. These values comprised 70%, 84%, and 89%, respectively, of all retrogradely labeled neurons in these regions. 3) Fibers and terminals arising from the regions of each of the three adrenergic cell groups were labeled by local injections of the anterogradely transported plant lectin PHA-L. Each component projection was found to distribute in a very similar fashion and to mimic the overall distribution of PNMT-IR; differential projection patterns within the PVH or SO were not seen consistently following deposits in any of the individual adrenergic cell groups or at different rostrocaudal levels of any individual cell group. 4) A dual anterograde tracing (PHA-L)-immunohistochemical (PNMT) labeling method revealed an appreciable number of varicosities arising from the regions of C1, C2, and C3 cell groups to contain PNMT-IR. These results suggest that adrenergic inputs to the PVH and SO, while arising from distinct medullary cell groups and presumably relaying different types of sensory information, are in a position to influence similar groups of parvicellular neurosecretory and/or autonomic-related projection neurons.
运用顺行运输、逆行运输及免疫组织化学技术，对延髓头端C1、C2和C3肾上腺素能细胞群至室旁核（PVH）和视上核（SO）的神经输入组织进行了特征描述。结果如下：1）苯乙醇胺-N-甲基转移酶免疫反应性（PNMT-IR）纤维和终末分布于PVH小细胞部的所有部位；背侧和背内侧亚区接受的输入最为显著，外侧和腹内侧部分接受的输入最少。在PVH大细胞部和SO中始终发现稀疏的终末场。2）采用逆行运输-免疫组织化学联合方法，估计C1、C2和C3细胞群区域中对PVH的PNMT-IR神经支配有贡献的细胞数量和比例。平均而言，C1细胞群中有232±37个逆行标记细胞，C2细胞群中有73±32个，C3细胞群中有96±26个PNMT-IR染色呈阳性。这些值分别占这些区域所有逆行标记神经元的70%、84%和89%。3）通过局部注射顺行运输的植物凝集素PHA-L，标记来自三个肾上腺素能细胞群各自区域的纤维和终末。发现每个成分投射的分布方式非常相似，且与PNMT-IR的总体分布相似；在任何单个肾上腺素能细胞群中沉积或在任何单个细胞群的不同头尾水平沉积后，在PVH或SO内未始终观察到差异投射模式。4）双顺行示踪（PHA-L）-免疫组织化学（PNMT）标记方法显示，来自C1、C2和C3细胞群区域的大量曲张体含有PNMT-IR。这些结果表明，PVH和SO的肾上腺素能输入虽然来自不同的延髓细胞群，且可能传递不同类型的感觉信息，但能够影响相似的小细胞神经分泌和/或自主神经相关投射神经元群。
