Department of Pathology and Diagnostics, University of Verona, Verona, Italy.
Leuk Lymphoma. 2013 Aug;54(8):1747-55. doi: 10.3109/10428194.2012.758843. Epub 2013 Jan 23.
Mantle cell lymphoma shows a peculiar immunogenetic profile, but the functional consequences of this fact are unknown. We have determined the precise sequences of rearranged heavy and light chain genes in several mantle cell lymphoma cell lines and investigated the presence of heavy and light chain stereotypy. These cell lines use IGHV and IGLV genes that are known to be preferentially rearranged in mantle cell lymphoma, but we found no evidence of heavy chain stereotypy. In contrast, one cell line (Mino) showed a nearly identical light chain complementarity-determining region 3 when compared to the only published light chain cluster. Two cell line couples (Jeko-1/UPN-2 and JVM-2/JVM-13) showed a highly similar light chain that satisfied the criteria for stereotypy. Our data show that mantle cell lymphoma cell lines resemble the IGHV and IGLV usage of mantle cell lymphoma, and foster the hypothesis that light chain stereotypy might be under-recognized.
套细胞淋巴瘤表现出一种特殊的免疫遗传学特征，但这一事实的功能后果尚不清楚。我们已经确定了几个套细胞淋巴瘤细胞系中重链和轻链基因重排的精确序列，并研究了重链和轻链的定型现象。这些细胞系使用IGHV 和 IGLV 基因，这些基因已知在套细胞淋巴瘤中优先重排，但我们没有发现重链定型的证据。相比之下，一个细胞系（Mino）与唯一发表的轻链簇相比，其轻链互补决定区 3 几乎完全相同。两个细胞系对（Jeko-1/UPN-2 和 JVM-2/JVM-13）显示出高度相似的轻链，符合定型的标准。我们的数据表明，套细胞淋巴瘤细胞系类似于套细胞淋巴瘤的 IGHV 和 IGLV 使用情况，并提出了轻链定型可能被低估的假设。
