Department of Chemical Safety and Toxicology, Bavarian Health and Food Safety Authority, Pfarrstrasse 3, D-80538 Munich, Germany.
Food Chem Toxicol. 2013 Mar;53:272-80. doi: 10.1016/j.fct.2012.12.004. Epub 2012 Dec 13.
Phthalates as well as di-(2-ethylhexyl) adipate (DEHA) are used as plasticizers in diverse applications and are of toxicological concern. The study was conducted with a study population of 25 German subjects aged between 15 and 21 months. Overall, 16 phthalates and DEHA were measured by gas chromatography-mass spectrometry in a total of 171 duplicate diet samples collected over 7 consecutive days, and 20 phthalate metabolites were analyzed in the urine samples collected over 7 consecutive days using a liquid chromatography-tandem mass spectrometry method. The median "high" daily dietary intake based on 95th percentiles was 4.66 μg/kg b.w. for di-2-ethylhexyl phthalate (DEHP), 1.03 μg/kg b.w. for di-isobutyl phthalate (DiBP), and 0.70 μg/kg b.w. for di-n-butyl phthalate (DnBP), and 1.0 μg/kg b.w. for DEHA. The "high" daily total intake from biomonitoring data was 4.9 μg/kg b.w. for DEHP, 2.2 μg/kg b.w. for DnBP, 3.9 μg/kg b.w. for DiBP, and 2.6 μg/kg b.w. for di-isononyl phthalate. The comparison of the two intake estimates indicates that the dominant intake source of DEHP was food ingestion, whereas other sources considerably contributed to the total intake of other phthalates. Using our "high" intake scenario, none of the analyzed phthalates reached the recommended tolerable daily intake levels.
邻苯二甲酸酯和己二酸二（2-乙基己基）酯（DEHA）被用作各种应用中的增塑剂，具有毒理学关注。该研究的研究对象为 25 名年龄在 15 至 21 个月之间的德国受试者。总体而言，通过气相色谱-质谱法在 7 天连续采集的 171 份重复饮食样本中测量了 16 种邻苯二甲酸酯和 DEHA，并使用液相色谱-串联质谱法在 7 天连续采集的尿液样本中分析了 20 种邻苯二甲酸代谢物。基于 95%百分位值，中位数“高”日膳食摄入量为：二（2-乙基己基）邻苯二甲酸酯（DEHP）4.66μg/kg bw，二异丁基邻苯二甲酸酯（DiBP）1.03μg/kg bw，二正丁基邻苯二甲酸酯（DnBP）0.70μg/kg bw，DEHA1.0μg/kg bw。生物监测数据“高”日总摄入量为：DEHP4.9μg/kg bw，DnBP2.2μg/kg bw，DiBP3.9μg/kg bw，邻苯二甲酸二异壬酯 2.6μg/kg bw。两种摄入量估计值的比较表明，DEHP 的主要摄入源是食物摄入，而其他来源对其他邻苯二甲酸的总摄入量有相当大的贡献。使用我们的“高”摄入量方案，没有一种分析的邻苯二甲酸酯达到推荐的可耐受日摄入量水平。
