Agronomy Department, Centre for Soil and Environmental Science, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
Environ Pollut. 2013 Mar;174:63-70. doi: 10.1016/j.envpol.2012.10.027. Epub 2012 Dec 14.
The effect of remediation using three EDTA doses (10, 30, 60 mmol kg(-1)) on soil functioning was assessed using column experiment and Brassica rapa. Soil washing removed up to 77, 29 and 72% of metals from soil contaminated with 1378, 578 and 8.5 mg kg(-1) of Pb, Zn and Cd, respectively. Sequential extraction indicated removal from the carbonate soil fraction. Metal oral-accessibility from the stomach phase was reduced by up to 75 and from the small intestine by up to 79% (Pb). Part of metals (up to 0.8% Cd) was lost due to leaching from columns. Remediation reduced toxic metal soil-root transfer by up to 61% but did not prevent metal accumulation in leaves. The fitness of plants grown on EDTA washed soils (gas exchange, fluorescence) was not compromised. Remediation initially reduced the soil DNA content (up to 29%, 30 mmol kg(-1) EDTA) and changed the structure of microbial population.
采用柱实验和油菜（Brassica rapa）研究了用三种 EDTA 剂量（10、30、60mmolkg(-1)）修复对土壤功能的影响。土壤洗涤可去除受污染土壤中分别为 1378、578 和 8.5mgkg(-1)的 Pb、Zn 和 Cd 的 77%、29%和 72%。连续提取表明金属从碳酸盐土壤部分被去除。金属口服可从胃相中减少高达 75%，从小肠相中减少高达 79%（Pb）。部分金属（高达 0.8%的 Cd）因从柱中淋洗而损失。修复将毒性金属从土壤向根转移减少了高达 61%，但不能防止金属在叶片中的积累。在经 EDTA 洗涤的土壤中生长的植物的适应性（气体交换、荧光）没有受到损害。修复最初会降低土壤 DNA 含量（高达 29%，用 30mmolkg(-1) EDTA）并改变微生物种群的结构。
