Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
Comp Biochem Physiol C Toxicol Pharmacol. 2013 Mar;157(2):251-7. doi: 10.1016/j.cbpc.2012.12.001. Epub 2012 Dec 13.
Prostaglandins are important regulators of reproductive function in fish. Analgesics like aspirin and ibuprofen are prostaglandin inhibitors and have been detected in freshwater systems at ng/L-μg/L levels. We investigated whether ibuprofen would affect prostaglandin and sex steroid hormone levels in adult zebrafish (Danio rerio) and if expression levels of genes involved in steroidogenesis and prostaglandin synthesis were affected. Zebrafish were exposed to moderate concentrations of ibuprofen (21, 201 or 506 μg/L) for 7 days in a semi-static test system. Ibuprofen concentrations were close to nominal levels and decreased by a maximum of 12-13% over 24 h. Prostaglandin E(2) (PGE(2)) levels in whole body homogenates of males and ovaries of females decreased in a monotonic dose-response relationship whereas male 11-ketotestosterone levels and ovarian 17β-estradiol levels remained unchanged. Ibuprofen did not have an influence on vitellogenin levels, female gonadosomatic index or cumulative egg production and no dose-response relationship in ovarian and testicular expression levels of the investigated genes was observed. This study shows that ibuprofen reduces PGE(2) levels in male and female zebrafish but has no consistent effects on other investigated reproductive parameters.
前列腺素是鱼类生殖功能的重要调节剂。像阿司匹林和布洛芬这样的镇痛药是前列腺素抑制剂，已在淡水系统中以 ng/L-μg/L 水平检测到。我们研究了布洛芬是否会影响成年斑马鱼（Danio rerio）的前列腺素和性激素水平，以及参与类固醇生成和前列腺素合成的基因的表达水平是否受到影响。在半静态测试系统中，斑马鱼暴露于中等浓度的布洛芬（21、201 或 506 μg/L）中 7 天。布洛芬浓度接近标称水平，在 24 小时内最多下降 12-13%。雄性全身体匀浆和雌性卵巢中前列腺素 E2（PGE2）水平呈单调剂量反应关系下降，而雄性 11-酮睾酮水平和卵巢 17β-雌二醇水平保持不变。布洛芬对卵黄蛋白原水平、雌性性腺指数或累积产卵量没有影响，也没有观察到卵巢和睾丸中研究基因表达水平的剂量反应关系。本研究表明，布洛芬降低了雄性和雌性斑马鱼的 PGE2 水平，但对其他研究的生殖参数没有一致的影响。
