Crankshaw D J, Challis J R, Patrick J E, Daniel E E
Gynecol Obstet Invest. 1979;10(6):298-305. doi: 10.1159/000299981.
Fractionation of pregnant sheep myometrium has been carried out and the fractions obtained analysed for their marker enzyme content and Ca2+ transporting properties. A fairly pure mitochondrial fraction and a very pure plasma membrane fraction were obtained. Both fractions could accumulate Ca2+ in the presence of ATP but the mechanisms were different, mitochondrial uptake had a high capacity and was inhibited by azide. Plasma membrane uptake was of low capacity and unaffected by azide. The effect of oxalate on plasma membrane suggests that there is an outwardly directed Ca2+ pump in the myometrial cell membrane. Both mitochondria and plasma membrane may be important in the Cas2+ accumulation of relaxation in this muscle.
对怀孕绵羊的子宫肌层进行了分级分离，并对所得级分进行了标记酶含量和钙离子转运特性分析。获得了一个相当纯的线粒体级分和一个非常纯的质膜级分。两个级分在ATP存在的情况下都能积累钙离子，但机制不同，线粒体摄取能力高且受叠氮化物抑制。质膜摄取能力低且不受叠氮化物影响。草酸盐对质膜的作用表明，子宫肌细胞膜中存在外向性钙离子泵。线粒体和质膜在该肌肉舒张过程中的钙离子积累中可能都很重要。
