Division of Bioengineering and Bioinformatics, Graduate School of Information Science and Technology, Hokkaido University, North 14, West 9, Sapporo, 060-0814, Japan.
Anal Chem. 2013 Jan 15;85(2):985-90. doi: 10.1021/ac302710m. Epub 2012 Dec 28.
This Article describes the simultaneous imaging of chiral nitroxyl radicals using electron paramagnetic resonance (EPR). Chiral nitroxyl radicals could be simultaneously visualized with the labeling of isotopic nitrogen. Chiral nitroxyl radicals, hydroxylmethyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl, were visualized using the method of simultaneous EPR imaging, which refers to the visualization of two kinds of molecules with unpaired electrons in a single image scan. EPR spectra of a racemic mixture of chiral nitroxyl radicals and those of the respective R and S configurations confirmed labeling by isotopic nitrogen. (1)H nuclear magnetic resonance (NMR) imaging and simultaneous imaging of solutions of chiral nitroxyl radicals were performed. The advantages and limitations of simultaneous imaging using EPR are also discussed. Simultaneous imaging with chiral-labeled nitroxyl radicals is a new application of EPR imaging and may be useful for biological studies involving biologically active chiral molecules.
本文描述了使用电子顺磁共振（EPR）同时对手性氮氧自由基进行成像。通过同位素氮的标记，可以同时可视化手性氮氧自由基。使用电子顺磁共振同时成像的方法可以可视化两种具有不成对电子的分子，这是在单次图像扫描中完成的。手性氮氧自由基的外消旋混合物的 EPR 光谱以及各自的 R 和 S 构型的 EPR 光谱证实了同位素氮的标记。还进行了手性氮氧自由基溶液的 1H 核磁共振（NMR）成像和同时成像。同时还讨论了使用 EPR 进行同时成像的优缺点。用手性标记的氮氧自由基进行同时成像，是 EPR 成像的一个新应用，可能对涉及生物活性手性分子的生物学研究有用。
