Maes A, Anthonissen R, Verschaeve L
Scientific Institute of Public Health, O.D. Public Health and Surveillance, Laboratory of Toxicology, Brussels, Belgium.
Folia Biol (Praha). 2012;58(5):215-20.
In order to evaluate the applicability of the cytokinesis-block micronucleus cytome assay in routine mutagenicity testing we investigated with this method different chemicals having different mechanisms of action: non-mutagens, direct-acting basealtering mutagens, direct-acting cross-linking mutagens, clastogens including a radiomimetic chemical, indirect-acting spindle poisons and indirect-acting enzyme inhibitors. We looked at the presence of micronuclei as biomarkers for either the loss of chromosome fragments (clastogen) or the loss of a whole chromosome (aneugen), nucleoplasmic bridges as biomarkers for complex rearrangements (e.g., dicentric chromosomes) and nuclear buds as biomarkers for gene amplification. The cytome assay proved to be a suitable tool to investigate genetic effects of environmental agents and to provide insight into their working mechanisms as all chemicals tested showed the expected response.
为了评估胞质分裂阻滞微核细胞分析法在常规致突变性测试中的适用性，我们用该方法研究了具有不同作用机制的不同化学物质：非诱变剂、直接作用的碱基改变诱变剂、直接作用的交联诱变剂、包括放射模拟化学物质在内的断裂剂、间接作用的纺锤体毒物和间接作用的酶抑制剂。我们将微核的存在视为染色体片段丢失（断裂剂）或整条染色体丢失（非整倍体剂）的生物标志物，将核质桥视为复杂重排（如双着丝粒染色体）的生物标志物，将核芽视为基因扩增的生物标志物。由于所有测试的化学物质都显示出预期的反应，因此细胞分析法被证明是一种研究环境因子遗传效应并深入了解其作用机制的合适工具。
