Sasaki Y, Shinohara Y, Baba S
Tokyo College of Pharmacy, Japan.
J Pharm Sci. 1990 Feb;79(2):96-8. doi: 10.1002/jps.2600790203.
The application of a stable isotope coadministration technique for estimating the fraction (fm) of imipramine (IP) that is converted to desipramine (DMI) is described. Four healthy male subjects received 25 mg of IP-d4 hydrochloride orally with 25 mg of DMI hydrochloride. The plasma concentrations of IP-d4, DMI-d4, and DMI were determined by capillary gas chromatography-mass spectrometry-selected ion monitoring using d8 analogues as internal standards. The fm values, calculated from the ratio of the area under the plasma concentration-time curve of DMI-d4 to that of DMI, varied from 0.54 to 0.85.
描述了一种用于估计转化为去甲丙咪嗪（DMI）的丙咪嗪（IP）的分数（fm）的稳定同位素共给药技术的应用。四名健康男性受试者口服25mg盐酸IP-d4和25mg盐酸DMI。使用d8类似物作为内标，通过毛细管气相色谱-质谱选择离子监测法测定血浆中IP-d4、DMI-d4和DMI的浓度。根据DMI-d4与DMI的血浆浓度-时间曲线下面积之比计算得到的fm值在0.54至0.85之间变化。
