Department of Molecular Pathology, Shinshu University Graduate School of Medicine, Matsumoto, Japan.
Pathology. 2013 Feb;45(2):150-4. doi: 10.1097/PAT.0b013e32835c766d.
Warthin's tumour is composed of bilayered oncocytic epithelium and organised lymphoid stroma, which resembles mucosa-associated lymphoid tissue (MALT); however, the histogenesis of the lymphoid stroma is not fully understood. We hypothesised that lymphocytes consisting of the stroma are recruited via high endothelial venules (HEVs) by the mechanism operating in normal lymphocyte homing in secondary lymphoid organs. The aim of this study was to determine immunohistochemically the molecules expressed on these HEVs.
Tissue sections of Warthin's tumour (n = 10) were immunostained for vascular addressin-related antigens including peripheral lymph node addressin (PNAd) and mucosal addressin cell adhesion molecule 1 (MAdCAM-1). An L-selectin·IgM chimera in situ binding assay was also carried out. Triple immunostaining for PNAd, CD3, and CD20/CD79α was performed to determine which lymphocyte subsets are closely associated with these HEVs.
HEVs in the lymphoid stroma of Warthin's tumour express PNAd, which is detected by MECA-79 as well as recently developed monoclonal antibodies S1 and S2. These HEVs were bound by L-selectin·IgM chimeras in a calcium-dependent manner, and numbers of lymphocytes, particularly T cells, attached to these HEVs.
The lymphoid stroma of Warthin's tumour is most likely developed by lymphocytes recruited via HEVs.
Warthin 瘤由双层嗜酸性上皮和有组织的淋巴间质组成，类似于黏膜相关淋巴组织（MALT）；然而，淋巴间质的组织发生尚未完全阐明。我们假设，基质中的淋巴细胞是通过在次级淋巴器官中正常淋巴细胞归巢所涉及的机制，通过高内皮静脉（HEV）募集的。本研究的目的是通过免疫组织化学方法确定这些 HEV 上表达的分子。
对 10 例 Warthin 瘤组织切片进行血管地址素相关抗原的免疫染色，包括外周淋巴结地址素（PNAd）和黏膜地址素细胞黏附分子 1（MAdCAM-1）。还进行了 L-选择素·IgM 嵌合体原位结合测定。进行 PNAd、CD3 和 CD20/CD79α 的三重免疫染色，以确定哪些淋巴细胞亚群与这些 HEV 密切相关。
Warthin 瘤的淋巴间质中的 HEV 表达 PNAd，这可以通过 MECA-79 以及最近开发的单克隆抗体 S1 和 S2 检测到。这些 HEV 以钙依赖性方式与 L-选择素·IgM 嵌合体结合，并且许多淋巴细胞，特别是 T 细胞，附着在这些 HEV 上。
Warthin 瘤的淋巴间质很可能是通过募集的淋巴细胞通过 HEV 发育而来的。
