Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Nanchang University, Nanchang 330006, China.
Biotechnol Lett. 2013 Apr;35(4):515-22. doi: 10.1007/s10529-012-1115-0. Epub 2012 Dec 19.
The most common genetic variations in the human genome, single nucleotide polymorphisms (SNPs), are ideal biomarkers and are used extensively in disease research. Here we introduce a novel method of PCR-conformation-difference gel electrophoresis (PCR-CDGE) used for detecting SNPs. The principle of PCR-CDGE relies PCR products from different homozygous DNA samples showing dissimilar migration patterns upon PAGE due to their conformational differences. PCR products from heterozygous DNA samples may exhibit two or more bands in PAGE because of the existence of DNA homoduplexes and heteroduplexes. In this study, analysis of two SNPs showed that PCR-CDGE is an accurate, simple, rapid, low-cost, and high-throughput genotyping method that could be used in most laboratories.
人类基因组中最常见的遗传变异是单核苷酸多态性（SNPs），它们是理想的生物标志物，在疾病研究中得到了广泛应用。在这里，我们介绍一种新的 PCR-构象差异凝胶电泳（PCR-CDGE）方法，用于检测 SNPs。PCR-CDGE 的原理是基于不同纯合 DNA 样本的 PCR 产物在 PAGE 中由于构象差异而表现出不同的迁移模式。杂合 DNA 样本的 PCR 产物在 PAGE 中可能会出现两条或更多条带，这是因为存在 DNA 同源双链体和异源双链体。在这项研究中，对两个 SNPs 的分析表明，PCR-CDGE 是一种准确、简单、快速、低成本和高通量的基因分型方法，可在大多数实验室中使用。
