Center for Molecular Allergology, IDI-IRCCS, Via dei Monti di Creta 104, 00167 Rome, Italy.
Br J Dermatol. 2013 Jun;168(6):1205-14. doi: 10.1111/bjd.12161.
Bullous pemphigoid (BP) is an autoimmune skin disease in which patient autoantibodies react with BP180 and BP230 proteins. In addition to IgG, IgE has been shown to play a role in the disease.
To evaluate the feasibility of detecting IgE and IgG against the immunodominant BP180 NC16A domain (BP180) using a microarray system.
BP180 was immobilized on an experimental version of the ISAC(®) microarray (Exp96). The BP study group and the controls were all tested on the commercial ISAC 103 version and on the Exp96. IgG and IgE were measured in a single run. BP180 IgG and IgE results were compared with those using an enzyme-linked immunosorbent assay (ELISA).
All results obtained using the IgG ELISA on the 31 patients with BP were replicated with the ISAC IgG. Five of eight BP sera tested by ELISA showed similar results with ISAC IgE. Twenty-nine (94%) and 19 (61%) of the 31 patients with BP were IgG and IgE positive to BP180, respectively, whereas four (3%) and six (4%) of 138 normal donors were IgG and IgE positive, respectively. Interestingly, the levels of IgG against BP180 detected using the ISAC system were related to the disease severity. Patients with BP showed a peculiar profile of IgE recognition toward some groups of allergens, which was absent in a group of allergic individuals. A significant, higher prevalence of hen's egg recognition was observed in patients with BP who had specific IgE to BP180.
The present preliminary study indicates that the ISAC microarray system is suitable for detecting IgG and IgE autoantibodies in patients with BP. Notably, this system allows the assessment of IgE and IgG autoantibodies at the same time, could be employed for the detection of autoantibodies to other autoantigens, and allows profiling for specific IgE to allergens.
大疱性类天疱疮（BP）是一种自身免疫性皮肤病，患者的自身抗体与 BP180 和 BP230 蛋白发生反应。除 IgG 外，IgE 也被证明在疾病中发挥作用。
评估使用微阵列系统检测针对免疫显性 BP180 NC16A 结构域（BP180）的 IgE 和 IgG 的可行性。
将 BP180 固定在实验版的 ISAC（®）微阵列（Exp96）上。BP 研究组和对照组均在商业 ISAC 103 版和 Exp96 上进行测试。IgG 和 IgE 在一次运行中进行测量。将 BP180 IgG 和 IgE 结果与酶联免疫吸附测定（ELISA）进行比较。
使用 31 例 BP 患者的 IgG ELISA 获得的所有结果均在 ISAC IgG 上得到复制。用 ELISA 测试的 8 例 BP 血清中的 5 例显示出与 ISAC IgE 相似的结果。31 例 BP 患者中有 29 例（94%）和 19 例（61%）分别对 BP180 IgG 和 IgE 呈阳性，而 138 例正常供体中有 4 例（3%）和 6 例（4%）分别对 BP180 IgG 和 IgE 呈阳性。有趣的是，使用 ISAC 系统检测到的针对 BP180 的 IgG 水平与疾病严重程度相关。BP 患者对某些组过敏原的 IgE 识别呈现出特殊的模式，而在一组过敏个体中则不存在这种模式。在对 BP180 具有特异性 IgE 的 BP 患者中，鸡卵识别的患病率显著更高。
本初步研究表明，ISAC 微阵列系统适用于检测 BP 患者的 IgG 和 IgE 自身抗体。值得注意的是，该系统允许同时评估 IgE 和 IgG 自身抗体，可以用于检测其他自身抗原的自身抗体，并允许对过敏原的特异性 IgE 进行分析。
