Biotechnology Division, Defense Research and Development Establishment, Gwalior 474 002, India.
Microbiol Immunol. 2013 Feb;57(2):145-9. doi: 10.1111/1348-0421.12014.
Although all mammals, including humans, are vulnerable when they come into direct contact with infected animals, anthrax is primarily a disease of herbivorous animals. In countries like India, cutaneous anthrax is a public health problem in several regions. Hence, a simple and efficacious serodiagnostic assay for large scale surveillance of endemic populations is required. In the present study, a field-usable, qualitative ELISA was developed for serodiagnosis of human anthrax. Results are assessed on a visual basis and no sophisticated instruments are required. Anti-protective antigen (PA) IgG was determined by visual examination of ELISA results of 225 human serum samples (160 from healthy humans, 5 from PA vaccinated individuals and 60 from confirmed anthrax cases). Comparison of the ELISA results with the results obtained from optical density values showed compatible sensitivity and specificity. Assay sensitivity, specificity, and positive and negative predictive values were found to be 100%. The developed assay could be a very useful tool for serological diagnosis of anthrax infection in humans.
虽然所有哺乳动物，包括人类，在直接接触受感染动物时都容易受到感染，但炭疽主要是食草动物的疾病。在印度等国家，皮肤炭疽是几个地区的公共卫生问题。因此，需要一种简单有效的血清学诊断检测方法，以便对地方性人群进行大规模监测。在本研究中，开发了一种现场可用的定性 ELISA 方法，用于炭疽的血清学诊断。结果是通过视觉评估的，不需要复杂的仪器。通过对 225 个人类血清样本（160 份来自健康人群，5 份来自 PA 疫苗接种个体，60 份来自确诊炭疽病例）的 ELISA 结果进行视觉检查，确定了抗保护性抗原（PA）IgG。将 ELISA 结果与光密度值的结果进行比较，显示出兼容的敏感性和特异性。检测的敏感性、特异性、阳性和阴性预测值均为 100%。该检测方法可能是人类炭疽感染血清学诊断的非常有用的工具。
