Wu Xiaoyu, Wang Rong, Xie Hua, Wang Jianfeng, Yang Pei, Jia Zhengping, Zhang Qiang, Wang Xianhua
PLA Key Laboratory of the Plateau of the Environmental Damage Control, Lanzhou General Hospital, Lanzhou Command, Lanzhou 730050, China.
Se Pu. 2012 Aug;30(8):810-5. doi: 10.3724/sp.j.1123.2012.03043.
A new convenient synthetic method was developed for the preparation of restricted-access material belonging to the group of the internal surface reversed-phase supports, which can be used for the direct injection and analysis of complex biological samples such as plasma samples. The supports, owning the function of reversed-phase chromatography, have an alkyl-chain only to the internal surfaces of the porous silica. Polyvinyl alcoholic groups chemically bound to the external surfaces of the porous silica that has the ability of hydrophilicity. Its structure was characterized by elemental analysis and electron microscope observation. The reversed-phase chromatographic performance was evaluated by using different solute probes, including propranolol, atenolol, phenobarbital and carbamazepine. Meanwhile, the comparative study with Merck Company' s ADS columns was carried out to prove capability of removing protein. At the same time, the plasma sample of propranolol was applied for the determination by direct injections. The results showed that the new internal surface reversed-phase restricted-access material owned the capabilities of protein exclusion and reversed-phase stationary, which could come true the double functions of excluding biomacromolecules and enriching micromolecule analysis. It can be used as a pretreatment column, which has the ability of online and fast analyzing biological specimen by direct probe inlet.
开发了一种新的简便合成方法，用于制备属于内表面反相载体组的受限进样材料，该材料可用于直接进样和分析复杂生物样品，如血浆样品。这些载体具有反相色谱功能，仅在多孔硅胶的内表面有烷基链。聚乙烯醇基团化学键合到具有亲水性的多孔硅胶外表面。通过元素分析和电子显微镜观察对其结构进行了表征。使用不同的溶质探针，包括普萘洛尔、阿替洛尔、苯巴比妥和卡马西平，对反相色谱性能进行了评估。同时，与默克公司的ADS柱进行了对比研究，以证明其去除蛋白质的能力。同时，将普萘洛尔的血浆样品用于直接进样测定。结果表明，新型内表面反相受限进样材料具有蛋白质排阻和反相固定能力，可实现排除生物大分子和富集小分子分析的双重功能。它可用作预处理柱，具有通过直接探针进样在线快速分析生物样品的能力。
