Jia Cong-cong, Lin Lin, Liu Ni-ya, Zhang Xiao-jing, Zhang Jia-jia, Yang Xin-jun, Huang Chen-ping
School of Environmental Science and Public Health, Wenzhou Medical College, Wenzhou 325035, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2012 Oct;30(10):730-4.
To investigate the effect of lead exposure on the gene expression of fibroblast growth factor 3 (Fgf3) in zebrafish embryonic development and the mechanism of lead-induced embryonic developmental toxicity.
The embryos of zebrafish (wild types A and B) were exposed to lead acetate (PbAc) at the doses of 0, 0.1, 0.5, 2.5, and 12.5 µmol/L separately. Total RNA was extracted from each treatment group of zebrafish embryos at 8, 12, 16, 24, 36, 48, and 72 hours post fertilization (hpf). The total mRNA expression of Fgf3 was measured by real-time quantitative PCR. The spatial expression of Fgf3 in zebrafish embryos was determined by whole-mount in situ hybridization using synthesized Fgf3 RNA probe.
The mRNA expression of Fgf3 in each group peaked at 12 hpf (P < 0.01). With the increase in PbAc concentration, the mRNA expression of Fgf3 rose. Compared with the mRNA expression level of Fgf3 in the control group, the relative mRNA expression levels of Fgf3 in the 0.1, 0.5, 2.5, and 12.5 µmol/L PbAc exposure groups were 1.02 ± 0.24, 1.05 ± 0.26, 1.22 ± 0.46, and 1.25 ± 0.38, respectively, and the 2.5 and 12.5 µmol/L PbAc exposure groups showed significantly higher Fgf3 expression than the control group (P < 0.05). The whole-mount in situ hybridization results showed that Fgf3 expression occurred mainly in the head and tail in the early stage of embryonic development and in the midbrain, fin bud, and pharyngeal arch in the middle/late stage of embryonic development; there were the most significant regions and intensities of positive hybridization signals at 12 hpf; but no significant differences were found between the control group and exposure groups in the location and intensity of Fgf3 expression
Lead exposure can result in the upregulation of Fgf3 expression in zebrafish embryonic development, which might contribute to lead-induced embryonic developmental toxicity.
研究铅暴露对斑马鱼胚胎发育过程中成纤维细胞生长因子3（Fgf3）基因表达的影响以及铅诱导胚胎发育毒性的机制。
将斑马鱼（野生型A和B）胚胎分别暴露于剂量为0、0.1、0.5、2.5和12.5 μmol/L的醋酸铅（PbAc）中。在受精后8、12、16、24、36、48和72小时（hpf）从每个处理组的斑马鱼胚胎中提取总RNA。通过实时定量PCR测定Fgf3的总mRNA表达。使用合成的Fgf3 RNA探针通过全胚胎原位杂交确定Fgf3在斑马鱼胚胎中的空间表达。
每组中Fgf3的mRNA表达在12 hpf时达到峰值（P < 0.01）。随着PbAc浓度的增加，Fgf3的mRNA表达升高。与对照组中Fgf3的mRNA表达水平相比，0.1、0.5、2.5和12.5 μmol/L PbAc暴露组中Fgf3的相对mRNA表达水平分别为1.02±0.24、1.05±0.26、1.22±0.46和1.25±0.38，并且2.5和12.5 μmol/L PbAc暴露组的Fgf3表达明显高于对照组（P < 0.05）。全胚胎原位杂交结果显示，Fgf3表达主要发生在胚胎发育早期的头部和尾部以及胚胎发育中/后期的中脑、鳍芽和咽弓；在12 hpf时有最明显的阳性杂交信号区域和强度；但对照组和暴露组在Fgf3表达的位置和强度上未发现显著差异。
铅暴露可导致斑马鱼胚胎发育过程中Fgf3表达上调，这可能是铅诱导胚胎发育毒性的原因。
