Lin Zhi-Ying, Chen Xiao-Chun, Zhang Jing, Huang Tian-Wen
Department of Neurology, the Affiliated Union Hospital of Fujian Medical University, Fuzhou 350001, China.
Sheng Li Xue Bao. 2012 Dec 25;64(6):687-94.
The present study was to record and separate the voltage-gated calcium channel currents (VGCCs) in primary cultured hippocampal neurons. An improved method is described for efficient and stable recording of VGCCs from primary cultured hippocampal neurons. The procedure allows the obtained hippocampal neurons overcome the defects from acutely isolated hippocampal neurons and currents do not rundown apparently after 20 min of membrane rupture. Using whole-cell configurations of patch clamp technique, it is shown that the method does not damage the neuron membrane electrical properties, and successfully records a variety of VGCCs components in hippocampal neurons (such as L, N, P/Q, T, etc.).
本研究旨在记录并分离原代培养海马神经元中的电压门控钙通道电流（VGCCs）。本文描述了一种改进方法，用于高效、稳定地记录原代培养海马神经元中的VGCCs。该方法能使获得的海马神经元克服急性分离海马神经元的缺陷，并且在膜破裂20分钟后电流无明显衰减。采用膜片钳技术的全细胞模式，结果表明该方法不会损害神经元膜的电学特性，并成功记录了海马神经元中多种VGCCs成分（如L、N、P/Q、T等）。
