Abel Peter W, Jain Neha
Department of Pharmacology, Creighton University School of Medicine, Omaha, Nebraska, USA.
Curr Protoc Pharmacol. 2012 Dec;Chapter 4:Unit 4.5. doi: 10.1002/0471141755.ph0405s59.
α-Adrenoceptors mediate responses to activation of both peripheral sympathetic nerves and central noradrenergic neurons. They also serve as autoreceptors that modulate the release of norepinephrine (NE) and other neurotransmitters. There are two major classes of α-adrenoceptors, the α(1)- and α(2). Each class is subdivided into three subtypes: α(1A), α(1B), α(1D), and α(2A), α(2B), α(2C). Described in this unit are in vitro isolated tissue methods used to study α-adrenoceptor functions and to identify novel ligands for these receptors. Detailed protocols describing use of isolated tissues to study the various α(1)- and α(2)-adrenoceptor subtypes are provided.
α-肾上腺素能受体介导外周交感神经和中枢去甲肾上腺素能神经元激活后的反应。它们还作为自身受体调节去甲肾上腺素（NE）和其他神经递质的释放。α-肾上腺素能受体主要分为两类，即α(1)和α(2)。每一类又细分为三个亚型：α(1A)、α(1B)、α(1D)以及α(2A)、α(2B)、α(2C)。本单元介绍了用于研究α-肾上腺素能受体功能和鉴定这些受体新配体的体外分离组织方法。还提供了详细的实验方案，描述了使用分离组织研究各种α(1)和α(2)肾上腺素能受体亚型的方法。
