Laboratory of Analytical Biochemistry and Bio-separation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Analyst. 2013 Feb 21;138(4):1137-40. doi: 10.1039/c2an36373a.
A novel moving redox reaction boundary (MRRB) model was developed for studying electrophoretic behaviors of analytes involving redox reaction on the principle of moving reaction boundary (MRB). Traditional potassium permanganate method was used to create the boundary model in agarose gel electrophoresis because of the rapid reaction rate associated with MnO(4)(-) ions and Fe(2+) ions. MRB velocity equation was proposed to describe the general functional relationship between velocity of moving redox reaction boundary (V(MRRB)) and concentration of reactant, and can be extrapolated to similar MRB techniques. Parameters affecting the redox reaction boundary were investigated in detail. Under the selected conditions, good linear relationship between boundary movement distance and time were obtained. The potential application of MRRB in electromigration redox reaction titration was performed in two different concentration levels. The precision of the V(MRRB) was studied and the relative standard deviations were below 8.1%, illustrating the good repeatability achieved in this experiment. The proposed MRRB model enriches the MRB theory and also provides a feasible realization of manual control of redox reaction process in electrophoretic analysis.
开发了一种新的移动氧化还原反应边界（MRRB）模型，用于研究涉及氧化还原反应的分析物在移动反应边界（MRB）原理下的电泳行为。由于 MnO(4)(-)离子和 Fe(2+)离子的快速反应速率，传统的高锰酸钾法被用于琼脂糖凝胶电泳中创建边界模型。提出了 MRB 速度方程来描述移动氧化还原反应边界（V（MRRB））和反应物浓度之间的一般功能关系，并且可以外推到类似的 MRB 技术中。详细研究了影响氧化还原反应边界的参数。在所选择的条件下，获得了边界移动距离与时间之间的良好线性关系。在两种不同浓度水平下，在电迁移氧化还原反应滴定中进行了 MRRB 的潜在应用。研究了 V（MRRB）的精度，相对标准偏差低于 8.1%，表明实验中达到了良好的重现性。所提出的 MRRB 模型丰富了 MRB 理论，还为电泳分析中氧化还原反应过程的手动控制提供了可行的实现方式。
