Liu Hui-Hui, Liu Xu-Dong, Wang Yan-Jie, Guan Hong-Quan, Chai Ji-Yan, Zhao Jin-Ru, Wang De-Shan
College of Basic Medicine, Liaoning University of TCM, Shenyang 110847, China.
Zhongguo Zhen Jiu. 2012 Oct;32(10):919-24.
To explore the point specificity of eye-acupuncture and the mechanism of eye-acupuncture on diarrhea-predominant irritable bowel syndrome (D-IBS).
Forty male Wistar rats of SPF grade were randomly divided into a normal group, a model group, a eye-acupuncture point (AA) group and a non-point (NA) group. The D-IBS rat model was established with the combination methods of the chronic stress and binding limbs. The AA group was treated by acupuncture at "low energizer area", "large intestine area", "liver area" and "spleen area", and the NA group by acupuncture at 3 mm apart from the same points area mentioned above, and the normal group and the model group with no intervention. The rate of feces moisture content was detected. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the mRNA of aquaporin 8 (AQP 8) in colon. Protein expressions of the vasoactive intestinal peptide (VIP) and AQP 8 in colon were detected by SABC immunohistochemistry method.
Compared with normal group, the rate of feces moisture content at the 18th and 25th days, VIP protein in colon mucosa, myenteric nerve plexus and hypo-mucosa nerve plexus increased significantly (all P < 0.01), and AQP 8 mRNA in colon mucosa decreased significantly in model, AA and NA group (P < 0.05, P < 0.01); AQP 8 protein in colon mucosa decreased significantly in model group and NA group (both P < 0.01). Compared with model group, the rate of feces moisture content at the 25th day and VIP protein in colon mucosa decreased significantly (P < 0.01, P < 0.05), and AQP 8 mRNA and protein increased significantly (P < 0.05, P < 0.01) in AA group. Compared with AA group, the rate of feces moisture content at the 25th day and VIP protein in colon mucosa increased significantly (both P < 0.01), and AQP 8 mRNA and protein decreased significantly (both P < 0.01) in NA group.
Eye-acupuncture has a good therapeutic effect on D-IBS. It is suggested that one of the mechanism is relate to increase AQP 8 in colon tissue and restrain the expression of VIP. Non-point area of eye-acupuncture has no obviously therapeutic effect and so to illustrate the point specificity of eye-acupuncture.
探讨眼针的穴位特异性及眼针治疗腹泻型肠易激综合征（D-IBS）的作用机制。
将40只SPF级雄性Wistar大鼠随机分为正常组、模型组、眼针穴位（AA）组和非穴位（NA）组。采用慢性应激结合束缚四肢的方法建立D-IBS大鼠模型。AA组针刺“下焦区”“大肠区”“肝区”“脾区”，NA组针刺于上述相同穴位区旁开3 mm处，正常组和模型组不干预。检测粪便含水量。采用逆转录-聚合酶链反应（RT-PCR）检测结肠水通道蛋白8（AQP 8）的mRNA。采用SABC免疫组化法检测结肠血管活性肠肽（VIP）和AQP 8的蛋白表达。
与正常组比较，模型组、AA组和NA组第18天和第25天粪便含水量、结肠黏膜、肌间神经丛和黏膜下神经丛VIP蛋白均显著升高（均P<0.01），结肠黏膜AQP 8 mRNA显著降低（P<0.05，P<0.01）；模型组和NA组结肠黏膜AQP 8蛋白显著降低（均P<0.01）。与模型组比较，AA组第25天粪便含水量和结肠黏膜VIP蛋白显著降低（P<0.01，P<0.05），AQP 8 mRNA和蛋白显著升高（P<0.05，P<0.01）。与AA组比较，NA组第25天粪便含水量和结肠黏膜VIP蛋白显著升高（均P<0.01），AQP 8 mRNA和蛋白显著降低（均P<0.01）。
眼针治疗D-IBS疗效显著。其作用机制之一可能与增加结肠组织AQP 8、抑制VIP表达有关。眼针非穴位区无明显治疗作用，从而说明了眼针的穴位特异性。
