Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, 518 Ziyue Road, Shanghai, 200241, China.
J Proteome Res. 2013 Feb 1;12(2):729-42. doi: 10.1021/pr3007864. Epub 2013 Jan 18.
Protein phosphorylation is an important posttranslational modification in many organisms that regulates numerous cellular processes. However, it remains poorly characterized in schistosomes, the causative agent of schistosomiasis in humans and related animals. In the present study, we characterized phosphorylated proteins in different stages and sex of Schistosoma japonicum (S. japonicum) including schistosomula (14 days), adult females (35 days), and adult males (35 days) by a titanium dioxide (TiO(2)) based phosphoproteomic method. A total of 180 phosphopeptides were identified in 148 proteins. Our further studies revealed that heat shock protein 90 (Hsp90), one of the phosphoproteins codetected in the different stage and sex of schistosomes, may play an important role in the regulation of schistosome development by directly or indirectly interacting with other codetected signal molecules. Additionally, some phosphoproteins were shown to be detected in a gender-specific manner, and the expressions of these proteins were further validated either by immunohistochemistry or by real-time reverse transcription polymerase chain reaction (RT-PCR) at transcript levels between male and female schistosomes. In summary, these findings as well as the providing of an inventory of phosphoproteins are expected to provide new insights into schistosome development and sexual maturation and then may result in the development of novel interventions against schistosomiasis.
蛋白质磷酸化是许多生物体中一种重要的翻译后修饰，它调节着许多细胞过程。然而，在血吸虫中，这种翻译后修饰的特征仍未被很好地描述，血吸虫是人类和相关动物中血吸虫病的病原体。在本研究中，我们通过基于二氧化钛 (TiO(2)) 的磷酸蛋白质组学方法，对日本血吸虫（S. japonicum）的不同发育阶段和性别（包括 14 日龄的尾蚴、35 日龄的雌性成虫和 35 日龄的雄性成虫）中的磷酸化蛋白进行了表征。在 148 种蛋白质中鉴定出了 180 个磷酸肽。我们的进一步研究表明，热休克蛋白 90（Hsp90）是在不同发育阶段和性别中共同检测到的磷酸蛋白之一，它可能通过与其他共同检测到的信号分子直接或间接相互作用，在血吸虫的发育调控中发挥重要作用。此外，一些磷酸蛋白表现出性别特异性，这些蛋白质的表达在雄性和雌性血吸虫之间的转录水平上通过免疫组织化学或实时逆转录聚合酶链反应 (RT-PCR) 进一步验证。总之，这些发现以及磷酸蛋白的提供，有望为血吸虫的发育和性成熟提供新的见解，从而可能导致开发针对血吸虫病的新干预措施。
