Hillier S L, Rabe L K, Muller C H, Zarutskie P, Kuzan F B, Stenchever M A
Department of Obstetrics and Gynecology, University of Washington, Seattle.
Obstet Gynecol. 1990 May;75(5):800-4.
Bacteria can be isolated from most seminal fluid samples, but the significance of these microorganisms is uncertain because most men lack symptoms associated with bacterial infection of the reproductive tract. We obtained semen samples from 37 men attending a Special Infertility Clinic and assessed the relationship between seminal fluid microorganisms and seminal fluid analysis including sperm motility, morphology, and concentration; the numbers of polymorphonuclear leukocytes and other white blood cells; and the hamster zona-free oocyte sperm penetration assay. Aerobic and/or anaerobic bacteria were recovered from 36 of the 37 samples. One hundred eighty-eight isolates (113 aerobes, 74 anaerobes, and one yeast) were recovered, with a mean of 5.2 isolates per semen specimen. The microorganisms recovered from the samples included: coagulase-negative staphylococci (89%), viridans streptococci (65%), diphtheroids (86%), Peptostreptococcus sp (62%), Bacteroides sp (27%), Gardnerella vaginalis (19%), Lactobacillus sp (16%), Actinomyces sp (16%), Enterococcus (11%), and Veillonella (11%). Other microorganisms including group B streptococcus, Hemophilus, Escherichia coli, Mobiluncus, and Clostridium were each recovered from fewer than 10% of the specimens. When the microbiology of seminal fluid specimens with or without polymorphonuclear leukocytes was compared, the presence of polymorphonuclear leukocytes in the semen was not associated with the isolation of staphylococci (33 versus 25%), viridans streptococci (33 versus 28%), Bacteroides sp (17 versus 37%), or Peptostreptococcus (31 versus 33%) (P greater than .05 for each comparison). The proportion of semen samples yielding bacterial isolates was similar after categorization by normal motility (more than 60%), pyospermia (six or more leukocytes per 100 sperm), sperm concentration, morphology, and a normal sperm penetration assay (11% or more).(ABSTRACT TRUNCATED AT 250 WORDS)
大多数精液样本中都能分离出细菌，但这些微生物的意义尚不确定，因为大多数男性没有与生殖道细菌感染相关的症状。我们从37名前往特殊不孕不育诊所就诊的男性身上获取了精液样本，并评估了精液微生物与精液分析之间的关系，精液分析包括精子活力、形态和浓度；多形核白细胞和其他白细胞的数量；以及仓鼠去透明带卵母细胞精子穿透试验。37份样本中的36份培养出了需氧菌和/或厌氧菌。共分离出188株菌株（113株需氧菌、74株厌氧菌和1株酵母菌），每份精液标本平均分离出5.2株菌株。从样本中分离出的微生物包括：凝固酶阴性葡萄球菌（89%）、草绿色链球菌（65%）、类白喉杆菌（86%）、消化链球菌属（62%）、拟杆菌属（27%）、阴道加德纳菌（19%）、乳杆菌属（16%）、放线菌属（16%）、肠球菌（11%）和韦荣球菌属（11%）。其他微生物，包括B族链球菌、嗜血杆菌、大肠杆菌、动弯杆菌和梭菌，从不到10%的标本中分离得到。比较有无多形核白细胞的精液标本的微生物学情况时，精液中多形核白细胞的存在与葡萄球菌（33%对25%）、草绿色链球菌（(33%对28%）、拟杆菌属（17%对37%）或消化链球菌（31%对33%）的分离无关（每次比较P均大于0.05）。按正常活力（超过60%）、脓精症（每100个精子中有6个或更多白细胞）、精子浓度、形态以及正常精子穿透试验（11%或更高）分类后，产生细菌分离株的精液样本比例相似。（摘要截短于250字）
