• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

利用基因表达谱分析鉴定肝星状细胞的转录调节因子。

Employment of gene expression profiling to identify transcriptional regulators of hepatic stellate cells.

作者信息

Shimada Hideaki, Rajagopalan Lakshman E

机构信息

Inflammation Research Unit, Pfizer Global Research and Development, Pfizer Inc, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA.

出版信息

Fibrogenesis Tissue Repair. 2012 Jun 6;5(Suppl 1):S12. doi: 10.1186/1755-1536-5-S1-S12. eCollection 2012.

DOI:10.1186/1755-1536-5-S1-S12
PMID:23259668
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3368757/
Abstract

Activated hepatic stellate cells (HSC) play a central role in scar formation that leads to liver fibrosis. The molecular mechanisms underlying this process are not fully understood. Microarray and bioinformatics analyses have proven to be useful in identifying transcription factors that regulate cellular processes such as cell differentiation. Using oligonucleotide microarrays, we performed transcriptional analyses of activated human HSC cultured on Matrigel-coated tissue culture dishes. Examination of microarray data following Matrigel-induced deactivation of HSC revealed a significant down-regulation of myocardin, an important transcriptional regulator in smooth and cardiac muscle development. Thus, gene expression profiling as well as functional assays of activated HSC have provided the first evidence of the involvement of myocardin in HSC activation.

摘要

活化的肝星状细胞（HSC）在导致肝纤维化的瘢痕形成过程中起核心作用。该过程背后的分子机制尚未完全了解。微阵列和生物信息学分析已被证明有助于识别调节细胞分化等细胞过程的转录因子。我们使用寡核苷酸微阵列对在基质胶包被的组织培养皿上培养的活化人HSC进行了转录分析。对基质胶诱导的HSC失活后的微阵列数据检查显示，心肌肌动蛋白显著下调，心肌肌动蛋白是平滑肌和心肌发育中的一种重要转录调节因子。因此，活化HSC的基因表达谱分析以及功能测定首次提供了心肌肌动蛋白参与HSC活化的证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1650/3368757/ab40511bd60c/1755-1536-5-S1-S12-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1650/3368757/ab40511bd60c/1755-1536-5-S1-S12-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1650/3368757/ab40511bd60c/1755-1536-5-S1-S12-1.jpg

相似文献

1
Employment of gene expression profiling to identify transcriptional regulators of hepatic stellate cells.利用基因表达谱分析鉴定肝星状细胞的转录调节因子。
Fibrogenesis Tissue Repair. 2012 Jun 6;5(Suppl 1):S12. doi: 10.1186/1755-1536-5-S1-S12. eCollection 2012.
2
Gene expression profiling and functional assays of activated hepatic stellate cells suggest that myocardin has a role in activation.激活的肝星状细胞的基因表达谱和功能分析表明， myocardin 在激活中起作用。
Liver Int. 2010 Jan;30(1):42-54. doi: 10.1111/j.1478-3231.2009.02120.x. Epub 2009 Sep 28.
3
Myocardin and myocardin-related transcription factor-A synergistically mediate actin cytoskeletal-dependent inhibition of liver fibrogenesis.肌球蛋白结合转录因子 A 与肌球蛋白相关转录因子 A 协同介导肌动蛋白细胞骨架依赖性的肝纤维化抑制。
Am J Physiol Gastrointest Liver Physiol. 2020 Mar 1;318(3):G504-G517. doi: 10.1152/ajpgi.00302.2019. Epub 2020 Jan 13.
4
Myocardin related transcription factor A programs epigenetic activation of hepatic stellate cells.肌球蛋白相关转录因子 A 程序激活肝星状细胞的表观遗传。
J Hepatol. 2015 Jan;62(1):165-74. doi: 10.1016/j.jhep.2014.07.029. Epub 2014 Aug 7.
5
Microarray profiling of circular RNAs and the potential regulatory role of hsa_circ_0071410 in the activated human hepatic stellate cell induced by irradiation.环状RNA的微阵列分析以及hsa_circ_0071410在辐射诱导的活化人肝星状细胞中的潜在调控作用
Gene. 2017 Sep 20;629:35-42. doi: 10.1016/j.gene.2017.07.078. Epub 2017 Jul 31.
6
Regulation of hepatic stellate cell activation and growth by transcription factor myocyte enhancer factor 2.转录因子肌细胞增强因子2对肝星状细胞激活和生长的调控
Gastroenterology. 2004 Oct;127(4):1174-88. doi: 10.1053/j.gastro.2004.07.007.
7
The orphan nuclear receptor COUP-TFII coordinates hypoxia-independent proangiogenic responses in hepatic stellate cells.孤儿核受体 COUP-TFII 协调肝星状细胞中缺氧非依赖性的促血管生成反应。
J Hepatol. 2017 Apr;66(4):754-764. doi: 10.1016/j.jhep.2016.11.003. Epub 2016 Nov 17.
8
Cooperation of liver cells in health and disease.健康与疾病状态下肝细胞的协作。
Adv Anat Embryol Cell Biol. 2001;161:III-XIII, 1-151. doi: 10.1007/978-3-642-56553-3.
9
The histone demethylase Kdm4 suppresses activation of hepatic stellate cell by inducing MiR-29 transcription.组蛋白去甲基化酶 Kdm4 通过诱导 MiR-29 转录来抑制肝星状细胞的活化。
Biochem Biophys Res Commun. 2019 Jun 18;514(1):16-23. doi: 10.1016/j.bbrc.2019.04.105. Epub 2019 Apr 20.
10
Basement membrane-like matrix inhibits proliferation and collagen synthesis by activated rat hepatic stellate cells: evidence for matrix-dependent deactivation of stellate cells.基底膜样基质抑制活化的大鼠肝星状细胞的增殖和胶原蛋白合成：星状细胞基质依赖性失活的证据。
Matrix Biol. 2003 May;22(3):229-39. doi: 10.1016/s0945-053x(03)00017-9.

引用本文的文献

1
The cellular microenvironment and cytoskeletal actin dynamics in liver fibrogenesis.肝脏纤维化发生过程中的细胞微环境与细胞骨架肌动蛋白动力学
Biocell. 2022 May 18;46(9):2003-2007. doi: 10.32604/biocell.2022.020171.
2
Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation.锚定蛋白激酶磷酸化在酒精性肝损伤和肝星状细胞激活中的作用。
Am J Pathol. 2018 Mar;188(3):640-655. doi: 10.1016/j.ajpath.2017.11.017. Epub 2018 Jan 2.
3
FoxO3 an important player in fibrogenesis and therapeutic target for idiopathic pulmonary fibrosis.

本文引用的文献

1
The Necdin-Wnt pathway causes epigenetic peroxisome proliferator-activated receptor gamma repression in hepatic stellate cells.Necdin-Wnt 通路导致肝星状细胞中表观遗传过氧化物酶体增殖物激活受体 γ 的抑制。
J Biol Chem. 2010 Oct 1;285(40):30463-71. doi: 10.1074/jbc.M110.156703. Epub 2010 Jul 27.
2
Gene expression profiling and functional assays of activated hepatic stellate cells suggest that myocardin has a role in activation.激活的肝星状细胞的基因表达谱和功能分析表明， myocardin 在激活中起作用。
Liver Int. 2010 Jan;30(1):42-54. doi: 10.1111/j.1478-3231.2009.02120.x. Epub 2009 Sep 28.
3
Gene expression profiles during activation of cultured rat hepatic stellate cells by tumoral hepatocytes and fetal bovine serum.
FoxO3 是肝纤维化发病机制中的一个重要因子，也是特发性肺纤维化的一个治疗靶点。
EMBO Mol Med. 2018 Feb;10(2):276-293. doi: 10.15252/emmm.201606261.
4
Epimorphin alters the inhibitory effects of SOX9 on Mmp13 in activated hepatic stellate cells.表皮形态发生素改变了SOX9对活化肝星状细胞中Mmp13的抑制作用。
PLoS One. 2014 Jun 27;9(6):e100091. doi: 10.1371/journal.pone.0100091. eCollection 2014.
5
Extracellular matrix and liver disease.细胞外基质与肝脏疾病
Antioxid Redox Signal. 2014 Sep 1;21(7):1078-97. doi: 10.1089/ars.2013.5697. Epub 2014 Jan 8.
培养的大鼠肝星状细胞被肿瘤性肝细胞和胎牛血清激活过程中的基因表达谱。
J Cancer Res Clin Oncol. 2010 Feb;136(2):309-21. doi: 10.1007/s00432-009-0666-5.
4
Hepatic stellate cell: a star cell in the liver.肝星状细胞：肝脏中的一种星形细胞。
Int J Biochem Cell Biol. 2009 Aug-Sep;41(8-9):1639-42. doi: 10.1016/j.biocel.2009.03.001. Epub 2009 Mar 11.
5
Resolution of liver cirrhosis using vitamin A-coupled liposomes to deliver siRNA against a collagen-specific chaperone.使用维生素A偶联脂质体递送针对胶原蛋白特异性伴侣蛋白的小干扰RNA来解决肝硬化问题。
Nat Biotechnol. 2008 Apr;26(4):431-42. doi: 10.1038/nbt1396. Epub 2008 Mar 30.
6
Gene expression profiles during the activation of rat hepatic stellate cells evaluated by cDNA microarray.
Arch Pharm Res. 2007 Nov;30(11):1410-8. doi: 10.1007/BF02977365.
7
TGF-beta up-regulates serum response factor in activated hepatic stellate cells.转化生长因子-β上调活化肝星状细胞中的血清反应因子。
Biochim Biophys Acta. 2007 Dec;1772(11-12):1250-7. doi: 10.1016/j.bbadis.2007.10.006. Epub 2007 Nov 4.
8
Gene expression profiles during hepatic stellate cell activation in culture and in vivo.培养和体内肝星状细胞激活过程中的基因表达谱
Gastroenterology. 2007 May;132(5):1937-46. doi: 10.1053/j.gastro.2007.02.033. Epub 2007 Feb 21.
9
The forkhead transcription factor FoxO1 regulates proliferation and transdifferentiation of hepatic stellate cells.叉头转录因子FoxO1调节肝星状细胞的增殖和转分化。
Gastroenterology. 2007 Apr;132(4):1434-46. doi: 10.1053/j.gastro.2007.01.033. Epub 2007 Jan 25.
10
Inactivation of myocardin and p16 during malignant transformation contributes to a differentiation defect.恶性转化过程中心肌素和p16的失活导致分化缺陷。
Cancer Cell. 2007 Feb;11(2):133-46. doi: 10.1016/j.ccr.2006.11.022.