Department of Biotechnology, Fooyin University, Kaohsiung City, Taiwan, ROC.
Vaccine. 2013 Jan 30;31(6):867-72. doi: 10.1016/j.vaccine.2012.12.017. Epub 2012 Dec 20.
The performance of a new type of tide mode culture system was investigated in this study. This novel bioreactor provides two separated stages, liquid and gas, for cell growth requirements. The immobilized cells absorbed the nutrient from medium during the liquid stage and subsequently were exposed directly to fresh air to absorb oxygen during the gas stage. Operating with PK15 cells under optimal conditions, we obtained 2.3×10(9) cells in 500ml reactor. It is 30 times higher than the initial inoculum and about 11 times higher than the production by roller bottle. For the vaccine production of classical swine fever (CSFV), a high virus titer of 2.1×10(9) median tissue culture infective dose (TCID(50)) was yielded which provided exceed 300 doses per milliliter of CSFV solution. Therefore, this new cultural system performed well not only for cell production but also for virus yield. It should be a highly efficient production for the CSFV vaccine and have practical potential in other animal cell culture vaccine.
本研究考察了一种新型潮汐模式培养系统的性能。该新型生物反应器提供了两个独立的阶段，即液体和气体，以满足细胞生长的需求。固定化细胞在液体阶段从培养基中吸收营养物质，随后在气体阶段直接暴露于新鲜空气中吸收氧气。在最佳条件下用 PK15 细胞进行操作，我们在 500ml 反应器中获得了 2.3×10(9)个细胞。这是初始接种物的 30 倍，比滚瓶培养的产量高 11 倍左右。对于猪瘟（CSFV）疫苗的生产，产生了 2.1×10(9)中值组织培养感染剂量（TCID(50)）的高病毒滴度，每毫升 CSFV 溶液提供超过 300 剂。因此，这种新型培养系统不仅在细胞生产方面表现出色，而且在病毒产量方面也表现出色。它应该是一种高效的 CSFV 疫苗生产方法，在其他动物细胞培养疫苗方面具有实际潜力。
