Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, No. 188 Xingwan Rd, Nanchang 330004, China.
J Chromatogr A. 2013 Jan 11;1272:73-80. doi: 10.1016/j.chroma.2012.11.070. Epub 2012 Dec 3.
A quantitative characterization of Polysorbate 80 is crucial for its many applications. In this paper we report a quick RP-HPLC method for the quantitative determination of Polysorbate 80. The hydrolysis of Polysorbate 80 to release oleic acid and three types of polyethers was first carried out. A chromatographic method based on liquid chromatography at critical conditions (LCCC) was then developed for an endgroup-based separation of low-molecular-mass polyether. With this method the polyether, irrespective of its molecular-mass, is separated according to endgroups (functionality) due to interactions of the polar endgroups with the hydrophilic stationary phase. The different types of polyethers and oleic acid were identified using on-line electrospray ionization mass spectrometry and quantified by evaporative light scattering detection.
聚山梨酯 80 的定量特性对于其许多应用至关重要。在本文中，我们报告了一种快速反相高效液相色谱法（RP-HPLC），用于定量测定聚山梨酯 80。首先进行聚山梨酯 80 的水解以释放油酸和三种类型的聚醚。然后开发了一种基于临界条件下的液相色谱法（LCCC）的色谱方法，用于基于端基的低分子量聚醚的分离。使用该方法，由于极性端基与亲水固定相的相互作用，聚醚（无论其分子量如何）都根据端基（官能团）进行分离。使用在线电喷雾电离质谱法鉴定不同类型的聚醚和油酸，并通过蒸发光散射检测进行定量。
