Holden H T, Varesio L, Taniyama T, Puccetti P
Adv Exp Med Biol. 1979;121B:509-20. doi: 10.1007/978-1-4684-8914-9_45.
In studies on the functional activity of macrophages isolated from murine sarcoma virus (MSV)-induced tumors, we have found that these cells may suppress immune responses as well as act as effector cells against the tumor. Previously, we reported that macrophages from the tumor could inhibit the antitumor response by suppressing proliferation-dependent immune functions. Here, we demonstrate that macrophages can also suppress the production of migration inhibition factor (MIF) and macrophage activation factor (MAF), two lymphocyte activities that are independent of cell proliferation. Conversely, we and others have found that macrophages from the tumor can exert an antitumor, cytolytic effect. In this study, using 1 g velocity sedimentation separation techniques, we have been able to identify 2-3 subpopulations of cytolytic macrophages in regressing tumors but in progressing tumors, only the smallest subpopulation of macrophages was active. T cells appeared to be required for activation of macrophages within the tumor, since MSV tumors induced in athymic, nude mice did not contain cytolytic macrophages.
在对从小鼠肉瘤病毒（MSV）诱导的肿瘤中分离出的巨噬细胞的功能活性进行的研究中，我们发现这些细胞可能会抑制免疫反应，同时也可作为抗肿瘤效应细胞。此前，我们报道过肿瘤中的巨噬细胞可通过抑制依赖增殖的免疫功能来抑制抗肿瘤反应。在此，我们证明巨噬细胞还能抑制迁移抑制因子（MIF）和巨噬细胞激活因子（MAF）的产生，这两种淋巴细胞活性与细胞增殖无关。相反，我们和其他人发现肿瘤中的巨噬细胞可发挥抗肿瘤的细胞溶解作用。在本研究中，使用1g速度沉降分离技术，我们已能够在消退的肿瘤中鉴定出2 - 3个细胞溶解巨噬细胞亚群，但在进展期肿瘤中，只有最小的巨噬细胞亚群具有活性。肿瘤内巨噬细胞的激活似乎需要T细胞，因为在无胸腺裸鼠中诱导产生的MSV肿瘤不含细胞溶解巨噬细胞。
