创伤弧菌与海洋聚集体的整合及其随后被弗吉尼亚牡蛎的摄取。
Integration of Vibrio vulnificus into marine aggregates and its subsequent uptake by Crassostrea virginica oysters.
机构信息
The University of North Carolina, Charlotte, NC, USA.
出版信息
Appl Environ Microbiol. 2013 Mar;79(5):1454-8. doi: 10.1128/AEM.03095-12. Epub 2012 Dec 21.
Abstract
Marine aggregates are naturally forming conglomerations of larvacean houses, phytoplankton, microbes, and inorganics adhered together by exocellular polymers. In this study, we show in vitro that the bacterial pathogen Vibrio vulnificus can be concentrated into laboratory-generated aggregates from surrounding water. We further show that environmental (E-genotype) strains exhibit significantly more integration into these aggregates than clinical (C-genotype) strains. Experiments where marine aggregates with attached V. vulnificus cells were fed to oysters (Crassostrea virginica) resulted in greater uptake of both C and E types than nonaggregated controls. When C- and E-genotype strains were cocultured in competitive experiments, the aggregated E-genotype strains exhibited significantly greater uptake by oyster than the C-genotype strains.
摘要
海洋聚集体是由磷虾类管栖生物的房屋、浮游植物、微生物和无机物自然聚集形成的，由细胞外聚合物黏合在一起。在这项研究中，我们在体外表明，细菌病原体创伤弧菌可以从周围水中浓缩到实验室生成的聚集体中。我们进一步表明，环境（E 基因型）菌株比临床（C 基因型）菌株更显著地整合到这些聚集体中。将附着有创伤弧菌细胞的海洋聚集体喂给牡蛎（Crassostrea virginica）的实验表明，C 型和 E 型的摄取量都高于未聚集的对照组。当 C 型和 E 基因型菌株在竞争实验中共培养时，聚集的 E 基因型菌株被牡蛎摄取的量明显大于 C 基因型菌株。
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