Busev V M, Semen'kov P G
Biokhimiia. 1979 Nov;44(11):2048-52.
The dependence of Na,K-ATPase activity on concentrations of ATP, Na+, K+, Mg2+ and ouabain in the membrane preparations of crab gills was studied. The first group of crabs was adapted to freshened (25%) and the second one--to normal (100%) sea water. A 40-day adaptation of crabs to the freshened sea water results in an increase of maximal activity of Na,K-ATPase, but does not affect the enzyme affinity for ATP, Na+, K+, Mg2+ and ouabain, as well as its cooperative properties. It is assumed that adaptation of crabs to freshened sea water is accompanied by an accumulation of Na, K-ATPase in the epithelial cell membranes or crab gills without causing any qualitative changes of the enzyme.
研究了蟹鳃膜制剂中钠钾ATP酶活性对ATP、Na⁺、K⁺、Mg²⁺和哇巴因浓度的依赖性。第一组螃蟹适应了淡化（25%）海水，第二组适应了正常（100%）海水。螃蟹对淡化海水40天的适应导致钠钾ATP酶最大活性增加，但不影响该酶对ATP、Na⁺、K⁺、Mg²⁺和哇巴因的亲和力及其协同特性。据推测，螃蟹对淡化海水的适应伴随着上皮细胞膜或蟹鳃中钠钾ATP酶的积累，而不会引起该酶的任何质性变化。
