University of Connecticut, Department of Chemistry, Storrs, CT 06269-3060, United States.
Curr Opin Chem Biol. 2013 Apr;17(2):137-42. doi: 10.1016/j.cbpa.2012.11.015. Epub 2012 Dec 25.
The first photocaged complexes appeared in the mid-1980s specifically to complement the ongoing work studying intracellular Ca(2+) signaling with fluorescent probes. Recently, the field of biological inorganic chemistry has focused increasingly on the homeostasis mechanisms and possible signaling functions of transition metal ions like Mn(2+), Fe(2+), Cu(+) and Zn(2+). Development of fluorescent sensors for imaging studies has been a very active area of research; however the corresponding chemical tools for delivering metal ions have received less interest. Using the roadmap provided by Ca(2+) predecessors, we describe how new photocaged complexes are being developed, and highlight some of the obstacles that must be overcome to investigate an emerging area in metal ion biology with different requirements.
第一代光笼复合物出现在 20 世纪 80 年代中期，主要是为了补充使用荧光探针研究细胞内 Ca(2+)信号转导的持续工作。最近，生物无机化学领域越来越关注过渡金属离子（如 Mn(2+)、Fe(2+)、Cu(+)和 Zn(2+)）的动态平衡机制和可能的信号功能。荧光传感器的发展用于成像研究一直是一个非常活跃的研究领域；然而，用于输送金属离子的相应化学工具却没有受到太多关注。我们根据 Ca(2+)的先驱物提供的路线图，描述了如何开发新的光笼复合物，并强调了在具有不同要求的金属离子生物学新兴领域进行研究时必须克服的一些障碍。
