通过定量聚合酶链反应诊断脑膜炎球菌感染：检测和定量脑膜炎奈瑟菌DNA

Diagnosis of meningococcal infection by QPCR: detection and quantification of DNA / Neisseria meningitidis.

作者信息

Nemescu Roxana Elena, Ursu Ramona Gabriela, Dorobăţ Carmen Mihaela, Iancu Luminiţa Smaranda

机构信息

Discipline of Infectious Diseases, University of Medicine and Pharmacy, Grigore T. Popa-Iaşi School of Medicine.

出版信息

Rev Med Chir Soc Med Nat Iasi. 2012 Jul-Sep;116(3):888-92.

PMID:23272547

Abstract

We present the case of a patient with clinical signs of meningococcemia and negative bacterial culture. Microbiological diagnosis was possible only by testing with real time PCR technique the cerebrospinal fluid and blood samples. Both pathologic samples were positive for Neisseria meningitidis by this rapid and sensitive diagnostic method.

摘要

我们报告一例有脑膜炎球菌血症临床体征但细菌培养阴性的患者。仅通过实时PCR技术检测脑脊液和血液样本才得以进行微生物学诊断。通过这种快速且灵敏的诊断方法，两份病理样本均检测出脑膜炎奈瑟菌呈阳性。