College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, 310053, People's Republic of China.
Chin Med. 2012 Dec 29;7(1):28. doi: 10.1186/1749-8546-7-28.
Our study aims to determine whether response surface methodology can optimize the extraction of dietary fiber from Maixiansan.
A Box-Behnken design was employed to optimize the extraction parameters, including α-amylase concentration (X1: 0.3 - 0.5%), enzymolysis time (X2: 30 - 60 min) and NaOH content (X3: 1.0 - 5.0%), of dietary fiber from Maixiansan using an enzyme-alkali extraction technique.
The optimal technological conditions were as follows: α-amylase concentration: 0.4%; enzymolysis time: 45 min; NaOH content: 4.0%. Under these conditions, the extraction yield reached 57.14%, which was well consistent with the predicted models with a coefficient of determination (R2) of 0.9818. An evaluation of the anti-inflammatory activity indicated that Maixiansan was able to significantly inhibit dextran sodium sulfate-induced ulcerative colitis in rats by increasing the concentration of short-chain fatty acids (acetate, propionate and butyrate), among which the butyrate content was significantly higher in the Maixiansan group than in the other groups.
Our experiments showed that response surface methodology can optimize the extraction of dietary fiber from Maixiansan. Maixiansan could be explored as an anti-ulcerative colitis agent.
本研究旨在确定响应面法是否可以优化麦纤散中膳食纤维的提取。
采用 Box-Behnken 设计，利用酶碱提取技术，优化膳食纤维提取的工艺参数，包括α-淀粉酶浓度（X1：0.3-0.5%）、酶解时间（X2：30-60min）和 NaOH 含量（X3：1.0-5.0%）。
最佳工艺条件为：α-淀粉酶浓度：0.4%；酶解时间：45min；NaOH 含量：4.0%。在此条件下，提取率达到 57.14%，与预测模型拟合度较好，决定系数（R2）为 0.9818。抗炎活性评价表明，麦纤散能显著增加大鼠短链脂肪酸（乙酸盐、丙酸盐和丁酸盐）的浓度，从而抑制葡聚糖硫酸钠诱导的溃疡性结肠炎，其中丁酸盐含量在麦纤散组明显高于其他组。
实验表明响应面法可优化麦纤散中膳食纤维的提取，麦纤散可作为一种抗溃疡性结肠炎药物进行探索。
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