Mohammadi Reza, Mohammadifar Mohammad Amin, Mortazavian Amir Mohammad, Rouhi Milad, Ghasemi Jahan B, Delshadian Zohre
Students Research Committee, Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences, Food Science and Technology, Shahid Beheshti University of Medical Sciences, P.O. Box 19395-4741, Tehran, Iran.
Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences, Food Science and Technology, Shahid Beheshti University of Medical Sciences, P.O. Box 19395-4741, Tehran, Iran.
Food Chem. 2016 Jan 1;190:186-193. doi: 10.1016/j.foodchem.2015.05.073. Epub 2015 May 19.
Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane. A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and analyzed by appropriate statistical methods. According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h. The experimental extraction yield under optimal conditions was found to be 30.049%, which is in good agreement with the predicted value of 30.054%.
采用响应面法（RSM）研究提取工艺变量对从蛋壳膜中提取胃蛋白酶可溶性胶原蛋白（PSC）的影响。采用中心复合设计（CCD）进行实验设计和结果分析，以获得氢氧化钠浓度（X1：0.4 - 1.2 mol/l）、碱处理时间（X2：6 - 30 h）、酶浓度（X3：15 - 75 U/mg）和水解时间（X4：12 - 60 h）的最佳组合，以实现最大程度的PSC提取。使用多元回归分析将获得的实验数据拟合到二阶多项式方程，并通过适当的统计方法进行分析。根据结果，最佳提取条件如下：氢氧化钠浓度为0.76 mol/l，碱处理时间为18 h，酶浓度为50 U/mg，水解时间为43.42 h。在最佳条件下的实验提取率为30.049%，与预测值30.054%吻合良好。
