Mirkin S M, Bogdanova E S, Gragerov A I, Larionov O A
Mol Biol (Mosk). 1979 Nov-Dec;13(6):1341-9.
A specific DNA-gyrase, inhibitor, coumermycin A1, causes differential changes in the spectrum of proteins synthesized in E. coli wild types cells, while protein spectrum in the mutant cells with coumermycin-resistant DNA-gyrase is left unaffected. The mutation of RNA-polymerase RpoB265 lowers the sensitivity of bacteria to coumermycin A1, whereas the RpoC3 enhances it. The differences between the normal and mutant RpoB265 RNA polymerases on interaction in vitro with ColE1 DNA plasmid depend on its supercoiling. No dependences of this kind were detected when comparing the normal and RpoC3-RNA polymerase. The obtained data indicate that the template supercoiling may significantly affect the transcription in vivo and that the properties of RNA polymerase may in some cases define this influence.
一种特定的DNA促旋酶抑制剂香豆霉素A1，会使大肠杆菌野生型细胞中合成的蛋白质谱发生差异变化，而具有抗香豆霉素DNA促旋酶的突变细胞中的蛋白质谱则不受影响。RNA聚合酶RpoB265的突变会降低细菌对香豆霉素A1的敏感性，而RpoC3的突变则会增强这种敏感性。正常的和突变的RpoB265 RNA聚合酶在体外与ColE1 DNA质粒相互作用时的差异取决于该质粒的超螺旋状态。在比较正常的和RpoC3-RNA聚合酶时，未检测到此类依赖性。所得数据表明，模板超螺旋可能会显著影响体内转录，并且在某些情况下，RNA聚合酶的特性可能决定这种影响。
