Department of Immunology and Allergology, National Center of Infectious and Parasitic Diseases, 26 Yanko Sakazov, Sofia, Bulgaria.
Diagn Microbiol Infect Dis. 2013 Mar;75(3):277-81. doi: 10.1016/j.diagmicrobio.2012.11.023. Epub 2012 Dec 28.
Current diagnostic standards for Mycobacterium tuberculosis (MTB) infection do not distinguish between active and latent tuberculosis (TB). To identify specific biomarkers characterizing the different forms of TB infection, we investigated in parallel with the QuantiFERON -TB Gold In-Tube (QFT-IT) the use of flow cytometry measuring CD4 and CD8 MTB-specific immune response in 17 active-TB patients, 21 health care workers (HCW), 14 recent contacts of TB patients (RC-TB), and 10 bacille Calmette Guerin (BCG)-vaccinated healthy controls (BCG-HC). A correlation (r = 0.4526, P = 0.0002) was found only between the amount of IFN-γ measured by QFT-IT and the frequency of CD4+/CD69+/IFN-γ+ T cells. The frequency of CD4+/CD69+/IFNγ+ responding T cells was higher in active-TB patients (0.254 ± 0.336%, P < 0.01) compared to the other groups. The response of QFT-IT antigen-specific CD8+/CD69+/IFNγ+ T cells was significantly higher in RC-TB (0.245 ± 0.305%, P < 0.05) compared to the other study groups.
目前用于结核分枝杆菌（MTB）感染的诊断标准无法区分活动性和潜伏性结核病（TB）。为了确定能够特异性描述不同形式 TB 感染的标志物，我们并行调查了 QFT-IT 法和流式细胞术检测 CD4 和 CD8 特异性 MTB 免疫应答，共纳入 17 例活动性 TB 患者、21 名医护人员（HCW）、14 例 TB 患者近期接触者（RC-TB）和 10 名卡介苗（BCG）接种健康对照者（BCG-HC）。仅发现 QFT-IT 检测的 IFN-γ量与 CD4+/CD69+/IFN-γ+T 细胞的频率之间存在相关性（r = 0.4526，P = 0.0002）。与其他组相比，活性 TB 患者的 CD4+/CD69+/IFNγ+反应性 T 细胞频率更高（0.254 ± 0.336%，P < 0.01）。与其他研究组相比，RC-TB 的 QFT-IT 抗原特异性 CD8+/CD69+/IFNγ+T 细胞的反应更高（0.245 ± 0.305%，P < 0.05）。
Zotero 插件