Laboratorio de Morfologia Celular, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón, CP. 11340 DF, México.
Clin Lymphoma Myeloma Leuk. 2013 Jun;13(3):266-72. doi: 10.1016/j.clml.2012.11.010. Epub 2012 Dec 29.
Epstein-Barr virus (EBV) is a member of the Herpesviridae family and is associated with Hodgkin lymphoma (HL). Isolates of EBV are classified according to sequence variation in the latency genes such as Epstein-Barr virus nuclear antigen (EBNA). EBNA2 contains the most divergent locus and is classified into type 1 and type 2 or EBNA2A and EBNA2B, respectively. We compared the frequency of EBV and the distribution of EBNA genotypes in Mexican children and adults with HL.
Lymph node biopsy specimens from children and adults with HL were embedded in paraffin. EBV was identified by LMP1 amplification and Epstein-Barr-encoded RNA EBER by in situ hybridization (ISH) and genotyped as EBNA2A or EBNA2B using nested polymerase chain reaction (PCR) and specific primers for the detection of subtype.
Sixty-six samples were obtained from 3 hospitals-42 (63%) from children and 24 (37%) from adults with HL. Thirty-two of the 42 samples (76.1%) were positive for EBV in children and 16 of 24 (66.6%) samples were positive in adults (P = .41). In both children and adults, EBV was found more frequently in male patients. Thirty-four of 48 cases could be typed (70.8%). EBNA2A was found in 7/21 (33.3%) children and in 4/13 (30.8%) adults (P = 1.0), and EBNA2B was found in 10/21 (47.6%) children and in 9/13 (69.2%) adults (P = .22). A mix of subtypes was found in 4/21 (19%) children.
EBV was found frequently in both children and adults with HL. EBNA2B was the most frequent subtype, and a high frequency of mixed subtypes was found in children.
爱泼斯坦-巴尔病毒（EBV）是疱疹病毒科的一员，与霍奇金淋巴瘤（HL）有关。根据潜伏基因（如 EBV 核抗原（EBNA））的序列变异，将 EBV 分离株分类。EBNA2 包含最具差异的基因座，并分为 1 型和 2 型或 EBNA2A 和 EBNA2B。我们比较了 EBV 在墨西哥儿童和成人 HL 中的频率以及 EBNA 基因型的分布。
将儿童和成人 HL 的淋巴结活检标本包埋在石蜡中。通过 LMP1 扩增和 EBV 编码 RNA EBER 的原位杂交（ISH）鉴定 EBV，并使用嵌套聚合酶链反应（PCR）和检测亚型的特异性引物将 EBV 基因型为 EBNA2A 或 EBNA2B。
从 3 家医院获得了 66 个样本-42 个（63%）来自儿童 HL，24 个（37%）来自成人 HL。42 个样本中的 32 个（76.1%）儿童 EBV 阳性，24 个样本中的 16 个（66.6%）成人 EBV 阳性（P =.41）。在儿童和成人中，EBV 在男性患者中更为常见。48 例中有 34 例可分型（70.8%）。在 21 例儿童中发现了 7 例（33.3%）EBNA2A 和 13 例成人中的 4 例（30.8%）（P = 1.0），在 21 例儿童中发现了 10 例（47.6%）EBNA2B 和 13 例成人中的 9 例（69.2%）（P =.22）。在 4 例儿童中发现了混合亚型。
在儿童和成人 HL 中均经常发现 EBV。EBNA2B 是最常见的亚型，儿童中发现了很高的混合亚型频率。
