Pajand O, Pourakbari B, Mahjob F, Aghamohammadi A, Mamishi N, Mamishi S
Department of Microbiology, Tabriz University of Medical Sciences, Tabriz, Iran.
Pediatr Hematol Oncol. 2011 Feb;28(1):10-5. doi: 10.3109/08880018.2010.507691. Epub 2010 Nov 17.
Hodgkin lymphoma (HL) is commonly associated with latent Epstein-Barr virus (EBV) infection. The aim of this study is a molecular analysis of the EBV status in both involved lymph node biopsies and plasma samples of patients with HL. Plasma and lymph node biopsy samples obtained from 15 pediatric and 10 adult HL patients were examined for EBV DNA using conventional polymerase chain reaction (PCR). The control group consisted of 30 healthy pediatrics and adults. In addition, immunoglobulin G (IgG) anti-EBV nuclear antigen (EBNA)-1 antibody was determined in sera of patients and controls using enzyme-linked immunosorbent assay (ELISA). IgG Anti EBNA-1 antibody was detected in 21 (84%) and 8 (26%) of patients and controls, respectively (P < .05). EBV DNA was detected in 12 (48%) and 1 (3%) plasma samples of patient and control cases, respectively. Significant difference was observed in plasma DNA detection between patients and controls (P < .05). Comparison of EBV DNA detection in plasma and biopsy samples between children and adult patients was only significant for plasma samples (P = .025). Significant correlation was observed in positive detection of EBV DNA between plasma and biopsy samples of the same individual (P < .001, r = .923). Frequency of EBV DNA in plasma and biopsy samples obtained from mixed-cellularity subgroup was higher than the nodular sclerosis; however, no significant difference was observed between these 2 subgroups. EBV detection in plasma of childhood Hodgkin lymphoma in a population with EBV seroconversion might be of value as a biomarker for EBV-associated Hodgkin lymphoma.
霍奇金淋巴瘤（HL）通常与潜伏性EB病毒（EBV）感染相关。本研究的目的是对HL患者受累淋巴结活检组织和血浆样本中的EBV状态进行分子分析。使用传统聚合酶链反应（PCR）检测从15例儿科和10例成人HL患者获得的血浆和淋巴结活检样本中的EBV DNA。对照组由30名健康儿童和成人组成。此外，使用酶联免疫吸附测定（ELISA）测定患者和对照组血清中的免疫球蛋白G（IgG）抗EB病毒核抗原（EBNA）-1抗体。分别在21例（84%）患者和8例（26%）对照组中检测到IgG抗EBNA-1抗体（P <.05）。分别在12例（48%）患者和1例（3%）对照病例的血浆样本中检测到EBV DNA。患者和对照组之间血浆DNA检测存在显著差异（P <.05）。儿童和成人患者血浆和活检样本中EBV DNA检测的比较仅在血浆样本中有显著差异（P =.025）。在同一个体的血浆和活检样本中，EBV DNA阳性检测之间存在显著相关性（P <.001，r =.923）。从混合细胞亚组获得的血浆和活检样本中EBV DNA的频率高于结节硬化型；然而，这两个亚组之间未观察到显著差异。在EBV血清学转换人群中，儿童霍奇金淋巴瘤血浆中的EBV检测可能作为EBV相关霍奇金淋巴瘤的生物标志物具有价值。
