Maruyama Nobuhiro, Segawa Tatsuya, Kinoshita Noriaki, Ichihara Atsuhiro
Immuno-Biological Laboratories Co, Ltd, Fujioka, Gunma, Japan.
Front Biosci (Elite Ed). 2013 Jan 1;5(2):583-90. doi: 10.2741/e640.
The (pro)renin receptor ((P)RR) plays a key role in the activation of the local renin-angiotensin system via interaction with renin and prorenin. A truncated form that is cleaved by furin, referred to as soluble (pro)renin receptor (s(P)RR), is secreted into the extracellular space. An accurate measurement of s(P)RR levels in vivo is an important issue in elucidating the roles of (P)RR in physiology and pathophysiology. To address this, we developed a sandwich ELISA that is applicable to human subjects. The standard curve of this ELISA showed a high linearity (125-8,000 pg/ml) with a correlation coefficient of >0.99. The recovery rate was approximately 90% in human blood and urine samples. The s(P)RR levels in plasma of healthy volunteers was in the range from 15.2 to 35.1 ng/ml (n = 20). Intra- and inter-assay coefficient of variations were less than 5.5% and 7.5%, respectively. It thus appears that this ELISA is a reliable tool for measuring s(P)RR levels in human subjects.
（前）肾素受体（(P)RR）通过与肾素和前肾素相互作用，在局部肾素 - 血管紧张素系统的激活中起关键作用。一种被弗林蛋白酶切割的截短形式，称为可溶性（前）肾素受体（s(P)RR），被分泌到细胞外空间。体内s(P)RR水平的准确测量是阐明(P)RR在生理学和病理生理学中作用的一个重要问题。为了解决这个问题，我们开发了一种适用于人类受试者的夹心ELISA。该ELISA的标准曲线具有高线性（125 - 8000 pg/ml），相关系数>0.99。在人血和尿样中的回收率约为90%。健康志愿者血浆中的s(P)RR水平在15.2至35.1 ng/ml范围内（n = 20）。批内和批间变异系数分别小于5.5%和7.5%。因此，这种ELISA似乎是测量人类受试者中s(P)RR水平的可靠工具。
