Hussels Martin, Konrad Alexander, Brecht Marc
Universität Tübingen, Institut für Physikalische und Theoretische Chemie and LISA+ Center, Auf der Morgenstelle 18, 72076 Tübingen, Germany.
Rev Sci Instrum. 2012 Dec;83(12):123706. doi: 10.1063/1.4769996.
The construction of a microscope with fast sample transfer system for single-molecule spectroscopy and microscopy at low temperatures using 2D/3D sample-scanning is reported. The presented construction enables the insertion of a sample from the outside (room temperature) into the cooled (4.2 K) cryostat within seconds. We describe the mechanical and optical design and present data from individual Photosystem I complexes. With the described setup numerous samples can be investigated within one cooling cycle. It opens the possibility to investigate biological samples (i) without artifacts introduced by prolonged cooling procedures and (ii) samples that require preparation steps like plunge-freezing or specific illumination procedures prior to the insertion into the cryostat.
报道了一种用于低温下单分子光谱和显微镜的具有快速样品转移系统的显微镜的构建，该系统使用二维/三维样品扫描。所展示的构建使得能够在几秒钟内将来自外部（室温）的样品插入冷却至（4.2 K）的低温恒温器中。我们描述了机械和光学设计，并展示了来自单个光系统I复合物的数据。利用所描述的装置，可以在一个冷却循环内研究大量样品。它为研究生物样品开辟了可能性：（i）不会因长时间冷却程序而引入伪像；（ii）对于那些在插入低温恒温器之前需要诸如骤冷或特定照明程序等制备步骤的样品。
