Caron John P, Gandy Jeff C, Schmidt Michelle, Hauptman Joseph G, Sordillo Lorraine M
Department of Large Animal, College of Veterinary Medicine, Michigan State University, East Lansing, MI, USA.
Vet Surg. 2013 Apr;42(3):231-7. doi: 10.1111/j.1532-950X.2012.01025.x. Epub 2012 Dec 20.
To compare the effects of triamcinolone acetonide (TA) and methylprednisolone acetate (MPA) on expression of selected chondrocyte genes in recombinant equine interleukin-1β (reIL-1β) stimulated articular cartilage explants.
In vitro experiment.
Horses (n = 6).
Articular cartilage explants from 2- to 3- year-old horses were exposed to reIL-1β in the presence and absence of TA and MPA at 10(-7) and 10(-6) M. Resting levels of mRNA of anabolic and catabolic genes of chondrocyte origin were quantified using qPCR after 6- and 12-hour incubations. Genes of interest included aggrecan interglobular domain, aggrecan, and collagen II, matrix metalloproteinases 3 and 13 (MMP3, MMP 13), aggrecanase 1, tissue inhibitor of matrix metalloproteinases 1 and 2 (TIMP 1, TIMP 2), BCL 2, vascular endothelial growth factor, and cyclooxygenase 2 (COX 2).
IL-1β significantly influenced the expression of most transcripts. MPA and TA inhibited the induction of MMP 13 at 6 and 12 hours; an effect that was significant at 6 hours with MPA at 10(-7) M and TA at 10(-6) M. Similarly, COX 2 was induced by reIL-1β and MPA and TA significantly inhibited its upregulation. TIMP 2 expression was reduced by reIL-1β, an effect that was significantly abrogated by MPA and TA. There were no significant differences observed between glucocorticoids for any gene studied.
No differential effects of MPA or TA on chondrocytic gene expression were identified suggesting that any divergent influences of these glucocorticoids on chondrocyte metabolism are posttranslational.
比较曲安奈德（TA）和醋酸甲泼尼龙（MPA）对重组马白细胞介素-1β（reIL-1β）刺激的关节软骨外植体中选定软骨细胞基因表达的影响。
体外实验。
马（n = 6）。
将2至3岁马的关节软骨外植体在有和没有10⁻⁷和10⁻⁶ M的TA和MPA存在的情况下暴露于reIL-1β。在孵育6小时和12小时后，使用qPCR对软骨细胞来源的合成代谢和分解代谢基因的mRNA静息水平进行定量。感兴趣的基因包括聚集蛋白聚糖球间结构域、聚集蛋白聚糖和胶原蛋白II、基质金属蛋白酶3和13（MMP3、MMP 13）、聚集蛋白聚糖酶1、基质金属蛋白酶组织抑制剂1和2（TIMP 1、TIMP 2）、BCL 2、血管内皮生长因子和环氧化酶2（COX 2）。
IL-1β显著影响大多数转录本的表达。MPA和TA在6小时和12小时时抑制MMP 13的诱导；在6小时时，10⁻⁷ M的MPA和10⁻⁶ M的TA这种作用显著。同样，reIL-1β诱导COX 2，MPA和TA显著抑制其上调。reIL-1β降低了TIMP 2的表达，MPA和TA显著消除了这种作用。在所研究的任何基因中，糖皮质激素之间均未观察到显著差异。
未发现MPA或TA对软骨细胞基因表达有差异影响，这表明这些糖皮质激素对软骨细胞代谢的任何不同影响都是翻译后水平的。
