通过色谱法和视频密度测定法测定酶活性。II. 组织匀浆中的尿素循环酶。

Determination of enzyme activity by chromatography and videodensitometry. II. Urea cycle enzymes in tissue homogenates.

作者信息

Karsai T, Ménes A, Molnár J, Elödi P

出版信息

Acta Biochim Biophys Acad Sci Hung. 1979;14(3):133-42.

Abstract

Methods are described for the determination of the activity of urea cycle enzymes in human and rat tissues by chromatography and videodensitometry(CV-technique). With specific substrates carbamoyl-phosphate synthetase and ornithine carbamoyltransferase activities were determined as the amounts of citrulline formed. Argininosuccinate synthetase, argininosuccinate lyase and arginase activities were measured from the changes in ornithine concentration. For measuring the activity of five enzymes 5 to 10 mg wet weight of tissue was sufficient. The CV-technique could be conveniently applied for the investigation of enzyme content in samples from human biopsy.

摘要

本文描述了通过色谱法和视频密度测定法（CV技术）测定人和大鼠组织中尿素循环酶活性的方法。使用特定底物，通过所形成瓜氨酸的量来测定氨甲酰磷酸合成酶和鸟氨酸氨甲酰转移酶的活性。根据鸟氨酸浓度的变化来测量精氨琥珀酸合成酶、精氨琥珀酸裂解酶和精氨酸酶的活性。对于测量这五种酶的活性，5至10毫克湿重的组织就足够了。CV技术可方便地用于研究人体活检样本中的酶含量。