PCR Hepatitis Laboratory, Department of Medicine, Maulana Azad Medical College and Associated Lok Nayak Hospital, University of Delhi, Delhi, India.
J Med Virol. 2013 Apr;85(4):620-6. doi: 10.1002/jmv.23508. Epub 2012 Dec 26.
The incidence and mortality in pregnant women with acute liver failure caused by hepatitis E virus (HEV) is high. Data on the viral load of HEV during pregnancy are limited. The study was designed to determine the viral load of HEV and its association with the disease severity in patients with acute liver failure. A total of HEV related 163 patients with acute liver failure which included 105 pregnant, 46 non-pregnant women and girls and 12 men and 730 patients with acute viral hepatitis which comprised of 220 pregnant women; 282 non-pregnant women and girls and 228 men were included. Viral load was measured by real-time PCR. Comparison was made between the pregnant and non-pregnant women. HEV RNA was detectable in 265 patients (142 pregnant; 75 non-pregnant and 48 men) and 104 patients with acute liver failure (64 pregnant, 34 non-pregnant and 6 men). The viral load of HEV in pregnant women with acute liver failure and acute viral hepatitis was significantly higher 129,984.0 ± 103,104.17 and 768.92 ± 1,105.40 copies/ml, respectively compared to the non-pregnant women which was 189.2 ± 225 and 12.73 ± 7.8 copies/ml (P < 0.0001). The viral load of HEV was also significantly higher in the pregnant patients with acute liver failure compared to the pregnant women with acute viral hepatitis and also men (P < 0.0001). High viral load of HEV during pregnancy could be one of the factors responsible for the severity of the infection during pregnancy.
戊型肝炎病毒（HEV）引起的孕妇急性肝衰竭的发病率和死亡率较高。关于妊娠期间 HEV 病毒载量的数据有限。本研究旨在确定 HEV 的病毒载量及其与急性肝衰竭患者疾病严重程度的关系。共纳入 163 例与 HEV 相关的急性肝衰竭患者，包括 105 例孕妇、46 例非孕妇和女孩以及 12 例男性；730 例急性病毒性肝炎患者，包括 220 例孕妇、282 例非孕妇和女孩以及 228 例男性。采用实时 PCR 检测病毒载量。比较了孕妇和非孕妇之间的差异。265 例患者（142 例孕妇；75 例非孕妇和 48 例男性）和 104 例急性肝衰竭患者（64 例孕妇、34 例非孕妇和 6 例男性）可检测到 HEV RNA。与非孕妇（189.2 ± 225 和 12.73 ± 7.8 拷贝/ml）相比，妊娠急性肝衰竭和急性病毒性肝炎孕妇的 HEV 病毒载量分别显著更高，分别为 129984.0 ± 103104.17 和 768.92 ± 1105.40 拷贝/ml（P < 0.0001）。与急性病毒性肝炎孕妇相比，妊娠急性肝衰竭孕妇的 HEV 病毒载量也显著更高，与男性相比也是如此（P < 0.0001）。妊娠期间 HEV 的高病毒载量可能是导致妊娠期间感染严重程度的因素之一。
