Franco Pilar, Zhang Tong
Chiral Technologies Europe, Illkirch, France.
Methods Mol Biol. 2013;970:113-26. doi: 10.1007/978-1-62703-263-6_6.
Owing to their remarkable enantioselectivity, versatility, and stability, immobilized polysaccharide-based chiral stationary phases (CSPs) have been successfully integrated into the tool box of many research and industry groups for the separation of enantiomers or stereoisomers by liquid and supercritical fluid chromatography. Due to the structurally diverse range of compounds available, efficient method development for chiral separations utilizing such CSPs is a challenging subject. In this chapter, we will discuss simplified screening protocols and straightforward approaches to achieve chiral separations in HPLC and SFC using the column series CHIRALPAK™ IA, IB, IC, and ID in reasonable time frame and with limited experimental work and a high success rate.
由于其卓越的对映体选择性、多功能性和稳定性，固定化多糖基手性固定相（CSPs）已成功纳入许多研究和工业团体的工具库，用于通过液相和超临界流体色谱法分离对映体或立体异构体。由于可用化合物的结构范围多样，利用此类CSPs进行手性分离的高效方法开发是一个具有挑战性的课题。在本章中，我们将讨论简化的筛选方案和直接的方法，以便在合理的时间范围内，通过有限的实验工作和高成功率，使用CHIRALPAK™ IA、IB、IC和ID柱系列在HPLC和SFC中实现手性分离。
