Li Chengke, Kang Jingwu
Chinese Academy of Sciences, Shanghai Institute of Organic Chemistry, Shanghai, China.
Methods Mol Biol. 2013;970:307-17. doi: 10.1007/978-1-62703-263-6_20.
Macrocyclic antibiotics, especially vancomycin, represent a class of versatile chiral selectors for enantioseparations by capillary electrophoresis (CE). In this chapter, we describe the protocol for performing CE enantioseparations with vancomycin as the chiral selector. Dynamic coating of the capillary with polymers including the positively charged polyelectrolyte hexadimethrine bromide or electro-neutral poly(dimethylacrylamide) proved to be very useful in order to reduce the adsorption of vancomycin onto the capillary wall resulting in an improved separation efficiency. The partial filling technique is employed for improvement of the detection sensitivity. Utilization of these techniques makes the CE enantioseparation with vancomycin more practical and robust.
大环抗生素，尤其是万古霉素，是一类用于毛细管电泳（CE）对映体分离的多功能手性选择剂。在本章中，我们描述了以万古霉素作为手性选择剂进行CE对映体分离的实验方案。用包括带正电荷的聚电解质溴化己二甲铵或电中性的聚（二甲基丙烯酰胺）在内的聚合物对毛细管进行动态涂层，被证明对于减少万古霉素在毛细管壁上的吸附非常有用，从而提高了分离效率。采用部分填充技术来提高检测灵敏度。这些技术的应用使使用万古霉素进行CE对映体分离更加实用和可靠。
