Sánchez-Hernández Laura, Domínguez-Vega Elena, Montealegre Cristina, Castro-Puyana María, Marina María Luisa, Crego Antonio L
Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, Faculty of Biology, Environmental Sciences and Chemistry, University of Alcalá, Alcalá de Henares (Madrid), Spain.
Electrophoresis. 2014 May;35(9):1244-50. doi: 10.1002/elps.201300489. Epub 2014 Jan 30.
The potential of the antibiotic vancomycin (VC) as chiral selector for the enantiomeric separation of amino acids by CE-ESI-MS/MS² was investigated for the first time in this work. Derivatization of amino acids with FMOC-Cl was carried out to enable their interaction with VC as well as the formation of precursor ions with larger m/z which were employed in MS² experiments. The partial filling of a coated capillary was employed to avoid the loss in MS sensitivity originated by the introduction of VC in the ionization source. Under optimized conditions, the simultaneous enantiomeric separation and unequivocal identification of 17 amino acids (two of them being nonprotein amino acids) took place in about 20 min with LODs in the micromolar range.
本工作首次研究了抗生素万古霉素（VC）作为手性选择剂通过CE-ESI-MS/MS²对氨基酸进行对映体分离的潜力。用FMOC-Cl对氨基酸进行衍生化，以使其能够与VC相互作用，并形成具有较大m/z的前体离子，这些前体离子用于MS²实验。采用涂覆毛细管的部分填充来避免由于在电离源中引入VC而导致的MS灵敏度损失。在优化条件下，17种氨基酸（其中两种为非蛋白质氨基酸）在约20分钟内实现了同时对映体分离和明确鉴定，检测限在微摩尔范围内。
