[评估显色培养基用于从腹泻儿童胃肠道中分离艰难梭菌的效用]
[Assessment of the usefulness of chromogenic medium for the isolation of Clostridium difficile from the gastrointestinal tract of children with diarrhoea].
作者信息
Kadzielska Joanna, Pituch Hanna, Banaszkiewicz Aleksandra, Radzikowski Andrzej, Wultańska Dorota, Obuch-Woszczatyński Piotr, Młynarczyk Grazyna
机构信息
Katedra i Zakład Mikrobiologii Lekarskiej Warszawski Uniwersytet Medyczny w Warszawie.
出版信息
Med Dosw Mikrobiol. 2012;64(3):197-201.
INTRODUCTION
Clostridium difficile is well known as an important cause of nosocomial infection. Laboratory diagnostics have included bacterial culture or more commonly, direct detection of preformed toxin in stool samples using different assays. The aim of this study was to evaluate and compare two selecitve media to isolation of C. difficile from paediatric diarrhoeal stool samples.
METHODS
Fifty nine stool samples, collected from 43 children with diarrhoea, were examined for routine laboratory diagnosis of C. difficile infection. Commercially available tests for detection of A/B toxins of C. difficile were performed. The same stool samples were cultured on two selective media for strain isolation: CLO and CDIFF (bioMerieux S.A., France) and incubated 48h and 24h respectively.
RESULTS
Twenty two samples gave positive results for toxins A/B C. difficile. From 24 samples inoculated on selective media C. difficile strains were cultured: from 8 samples on CLO medium and from 16 samples on CDIFF medium.
CONCLUSIONS
CDIFF medium is more effective for isolation of C. difficile strains from stool samples collected from children with diarrhoea.
引言
艰难梭菌是医院感染的重要病因，广为人知。实验室诊断方法包括细菌培养，或更常见的是使用不同检测方法直接检测粪便样本中预先形成的毒素。本研究的目的是评估和比较两种用于从儿科腹泻粪便样本中分离艰难梭菌的选择性培养基。
方法
从43名腹泻儿童中收集了59份粪便样本，用于艰难梭菌感染的常规实验室诊断。进行了检测艰难梭菌A/B毒素的市售检测。将相同的粪便样本接种在两种用于菌株分离的选择性培养基上：CLO和CDIFF（法国生物梅里埃公司），分别培养48小时和24小时。
结果
22份样本艰难梭菌毒素A/B检测呈阳性。在接种于选择性培养基的24份样本中培养出了艰难梭菌菌株：8份样本在CLO培养基上培养出，16份样本在CDIFF培养基上培养出。
结论
CDIFF培养基在从腹泻儿童收集的粪便样本中分离艰难梭菌菌株方面更有效。
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