Bhutia Tshering Doma, Valant-Vetschera Karin M, Adlassnig Wolfram, Brecker Lothar
Chemodiversity Research Group, Dept. of Systematic and Evolutionary Botany, Institute of Botany, University of Vienna, Rennweg 14, A-1030 Vienna, Austria.
Nat Prod Commun. 2012 Nov;7(11):1469-73.
A combined study was carried out on the micromorphology and chemistry of glandular trichomes with focus on Primula vialii and P. vulgaris, respectively. Epifluorescence microscopy was applied to study the auto-fluorescent properties of flavonoids and their localization in glandular trichomes. Both species differed in the morphology of the glandular trichomes and in the exudate flavonoid composition. Leaf glands from P. vialii and from some species of subgen. Primula exhibited uniform glandular fluorescence, but notable differentiation was observed within a single leaf of P. vulgaris. Our observations indicate that exudate flavonoids are not transported from the tissue to the glandular hairs. Conversely, only the newly isolated glycoside 1 (kaempferol 3-O-(2"'-rhamnosyl)-robinobioside) was obtained from leaf tissue of P. vialii after removal of the exudate. Its structure was confirmed by NMR and mass spectrometry. This glycoside was not detected in tissue extracts of P. vulgaris after similar treatment. The observed chemical diversity is discussed, with focus on possible correlation with glandular structures and tissue differentiation in Primula, and also against other studied species. Aspects of biosynthesis in relation to tissue-specific flavonoid diversification are shortly addressed.
分别以长柱报春花和报春花为重点，对腺毛的微观形态和化学组成进行了联合研究。应用落射荧光显微镜研究黄酮类化合物的自发荧光特性及其在腺毛中的定位。两种植物在腺毛形态和渗出物黄酮类化合物组成上存在差异。长柱报春花和报春花亚属某些物种的叶腺表现出均匀的腺荧光，但在报春花的单叶内观察到明显的分化。我们的观察表明，渗出物黄酮类化合物不会从组织转运到腺毛。相反，去除渗出物后，仅从长柱报春花的叶组织中获得了新分离的糖苷1（山奈酚3 - O -（2'''-鼠李糖基）-刺槐二糖）。其结构通过核磁共振和质谱得到证实。经过类似处理后，在报春花的组织提取物中未检测到这种糖苷。讨论了观察到的化学多样性，重点关注其与报春花中腺结构和组织分化的可能相关性，以及与其他研究物种的对比。简要阐述了与组织特异性黄酮类化合物多样化相关的生物合成方面。
