[线性未整合劳斯肉瘤病毒DNA片段，由SmaI限制性内切核酸酶消化产生]
[Fragments of linear unintegrated Rous sarcoma virus DNA, resulting from digestion with SmaI restriction endonuclease].
作者信息
Marx M, Kaczorek M, Cerutti P
出版信息
C R Seances Acad Sci D. 1979 Nov 26;289(14):1021-4.
PMID:232859
Abstract
Linear unintegrated DNA of Schmidt-Ruppin Rous sarcoma virus, subgroup D (SR-RSV-D), was digested with SmaI restriction endonuclease, analyzed by agarose gel electrophoresis, "blotted" by Southern's method and hybridized with a viral 32P cDNA. SmaI cleaves this DNA at five sites, two of which are localized at the ends of the provirus. Src and env genes seem not to be restrictied by SmaI cleavage.
摘要
施密特 - 鲁平罗氏肉瘤病毒D亚组（SR - RSV - D）的线性未整合DNA用SmaI限制性内切酶消化，通过琼脂糖凝胶电泳分析，用Southern法“印迹”，并用病毒32P cDNA杂交。SmaI在五个位点切割该DNA，其中两个位于前病毒的末端。Src和env基因似乎不受SmaI切割的限制。
Zotero 插件