[环孢素A和他克莫司对肾移植受者免疫基因表达的调节作用]

[Regulatory effects of cyclosporin A and tacrolimus on the immunological gene expressions in renal transplant recipients].

作者信息

Wen Jin, Ji Zhi-gang, Niu Ji-rui

机构信息

Department of Urology, PUMC Hospital, CAMS and PUMC, Beijing, China.

出版信息

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2012 Dec;34(6):563-6. doi: 10.3881/j.issn.1000-503X.2012.06.005.

DOI:10.3881/j.issn.1000-503X.2012.06.005

PMID:23286399

Abstract

OBJECTIVE

To observe the change of Th immunological gene in renal transplant recipients after the treatment of cyclosporine (CsA) and tacrolimus (FK506).

METHODS

The peripheral blood lymphacytes just before and 24 hours after CsA and FK506 treatment were isolated. The total RNA of them were reverse-transcripted and examined by real-time quantity PCR array. The results were analyzed by bioinformatic methods.

RESULTS

The TLR4, CEBPB, IL4R, IL1R1,IL18R1,and IL1R2 genes were remarkably upregulated, whereas IL-2, CCL5, CD27, CCR5, CCR4, CD4, RPL13A, TGFB3, CD86, CCR3, STAT1, NFATC2IP, IL23A, IL15, IRF4, and TFCP2 were downregulated 24 hours after CsA treatment. The IL18, IL7, PTPRC, TNFSF4, SPP1, GFI1, TLR4, IL13RA1, TNF, INHBA, LAG3, IL13, IL1R1, SOCS5, IL10, YY1, TBX21, FASLG, IL18R1, and IL1R2 genes were remarkably upregulated, whereas IL-2, IL-3, IL-4, IL-6,CCR5, CD4, CD27, CD40LG, IL15, CCR3, CD86, CCR4, and IRF4 were obviously downregulated 24 hours after FK506 treatment.

CONCLUSION

CsA and FK506 exert their therapeutic effectiveness by regulating the expressions of a series of target genes.

摘要

目的

观察环孢素（CsA）和他克莫司（FK506）治疗后肾移植受者Th免疫基因的变化。

方法

分离CsA和FK506治疗前及治疗后24小时的外周血淋巴细胞。提取其总RNA进行逆转录，并通过实时定量PCR芯片检测。采用生物信息学方法分析结果。

结果

CsA治疗24小时后，TLR4、CEBPB、IL4R、IL1R1、IL18R1和IL1R2基因显著上调，而IL-2、CCL5、CD27、CCR5、CCR4、CD4、RPL13A、TGFB3、CD86、CCR3、STAT1、NFATC2IP、IL23A、IL15、IRF4和TFCP2基因下调。FK506治疗24小时后，IL18、IL7、PTPRC、TNFSF4、SPP1、GFI1、TLR4、IL13RA1、TNF、INHBA、LAG3、IL13、IL1R1、SOCS5、IL10、YY1、TBX21、FASLG、IL18R1和IL1R2基因显著上调，而IL-2、IL-3、IL-4、IL-6、CCR5、CD4、CD27、CD40LG、IL15、CCR3、CD86、CCR4和IRF4基因明显下调。