Kokshoorn Bas, Blankers Bart J
Department of Human Biological Traces, Netherlands Forensic Institute, P,O, Box 24044, The Hague, NL-2490AA, the Netherlands.
Investig Genet. 2013 Jan 3;4(1):1. doi: 10.1186/2041-2223-4-1.
In a recent contribution to this journal Grisedale and Van Daal concluded that a single STR analysis of all available template DNA is to be preferred over replicate analyses and a consensus approach when analyzing low template DNA samples. A single STR analysis approach does not allow for an assessment of the validity of the resulting DNA profile. We argue that the use of replicate amplifications is the best way to objectively quantify the extent of the stochastic variation in the data. By applying consensus methodology and/or a probabilistic model, the interpretation of the data will therefore be more objective and reliable.
在近期发表于本期刊的一篇文章中，格里塞代尔和范·达尔得出结论，在分析低模板DNA样本时，对所有可用模板DNA进行单次STR分析比重复分析和采用共识方法更为可取。单次STR分析方法无法评估所得DNA图谱的有效性。我们认为，使用重复扩增是客观量化数据中随机变异程度的最佳方法。因此，通过应用共识方法和/或概率模型，数据的解释将更加客观和可靠。
